Search
Skip to Search Results-
2016
Richards, Michele R., Campuzano, Iain D. G., Kitova, Elena N., Klassen, John S., Li, Jun, Privé, Gilbert G., Xiong, Zi Jian, Bagal, Dhanashri
Saposin A (SapA) lipoprotein discs, also known as picodiscs (PDs), represent an attractive method to solubilize glycolipids for protein interaction studies in aqueous solution. Recent electrospray ionization mass spectrometry (ESI-MS) data suggest that the size and composition of...
-
2016
Klassen, John S., Campuzano, Iain D. G., Bagal, Dhanashri, Privé, Gilbert G., Kitova, Elena N., Xiong, Zi Jian, Richards, Michele R., Li, Jun
Saposin A (SapA) lipoprotein discs, also known as picodiscs (PDs), represent an attractive method to solubilize glycolipids for protein interaction studies in aqueous solution. Recent electrospray ionization mass spectrometry (ESI-MS) data suggest that the size and composition of...
-
Comparison of the Responsivity of Solution-Suspended and Surface-Bound Poly (N-isopropylacrylamide)-Based Microgels for Sensing Applications
Download2017-01-01
Li, Wenxiang, Hu, Liang, Zhu, Jinghua, Li, Dan, Luan, Yafei, Xu, Wenwen, Serpe, Michael J.
In this submission, the phase transition behavior for poly(N-isopropylacrylamide-co-acrylic acid) (pNIPAm-co-AAc) microgels and their assemblies was investigated as a function of temperature and pH using UV–vis spectroscopy (to probe light scattering behavior) and quartz crystal microbalance with...
-
Conformational analysis of galactofuranosides using NMR spectroscopy and computational chemistry
DownloadFall 2012
The five-membered ring, or furanose, form of galactose is not found in mammalian systems. However, it is commonly found in pathogenic organisms, including Aspergillus fumigatus, Campylobacter jejuni, and Mycobacterium tuberculosis. In many of these organisms, galactofuranose (Galf) is essential...
-
Fall 2016
This thesis describes the development of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was first applied to quantify protein-carbohydrate interactions in aqueous ammonium acetate...
-
Electrically Triggered Small Molecule Release from Poly (N-Isopropylacrylamide-co-Acrylic Acid) Microgel-Modified Electrodes
Download2018-01-01
Xu, Wenwen, Zhang, Y., Gao, Yongfeng, Serpe, Michael J.
A monolithic layer of poly(N-isopropylacrylamide-co-acrylic acid) microgels was deposited on an Au electrode and used for electrically triggered release of the small molecule crystal violet (CV), which was used as a model drug. CV was loaded into the surface-bound microgels by exposing them to a...
-
2014
Serpe, Michael J., Xu, Wenwen, Gao, Yongfeng
In this submission, pH responsive poly(N-isopropylacrylamide)–based microgels were used to fabricate optical devices (etalons). We demonstrate that the optical properties of the etalons could be manipulated upon the application of an appropriate electric potential between the etalon and a counter...
-
2014
Serpe, Michael J., Xu, Wenwen, Gao, Yongfeng
Poly (N-isopropylacrylamide-co-acrylic acid) (pNIPAm-co-AAc) microgel-based free-standing etalons have been developed by fabricating etalons on a solid support that has been coated with a sacrificial polymer layer. Therefore, the etalon could be easily removed from the solid substrate upon...
-
2016
Klassen, John S., Yao, Yuyu, Kitova, Elena N., Richards, Michele R.
The results of an investigation into the influence of sulfolane, a commonly used supercharging agent, on electrospray ionization mass spectrometry (ESI-MS) measurements of protein–ligand affinities are described. Binding measurements carried out on four protein–carbohydrate complexes, lysozyme...
-
2016
Klassen, John S., Yao, Yuyu, Kitova, Elena N., Richards, Michele R.
The results of an investigation into the influence of sulfolane, a commonly used supercharging agent, on electrospray ionization mass spectrometry (ESI-MS) measurements of protein–ligand affinities are described. Binding measurements carried out on four protein–carbohydrate complexes, lysozyme...