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Cardiomyocyte overexpression of iNOS in mice results in peroxynitrite generation, heart block, and sudden death
Download2002-01-01
Mungrue, Imran N., Gros, Robert, You, Xiaomang, Pirani, Asif, Azad, Azar, Csont, Tamas, Husain, Mansoor, Schulz, Richard
Increased inducible nitric oxide synthase (iNOS) expression is a component of the immune response and has been demonstrated in cardiomyocytes in septic shock, myocarditis, transplant rejection, ischemia, and dilated cardiomyopathy. To explore whether the consequences of such expression are...
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Doxycycline and benznidazole reduce the profile of Th1, Th2, and Th17 chemokines and chemokine receptors in cardiac tissue from chronic Trypanosoma cruzi-infected dogs
Download2016-01-01
Costa, Guilherme de Paula, Lopes, Laís Roquete, Silva, Maria Cláudia da, Horta, Aline Luciano, Pontes, Washington Martins, Milanezi, Cristiane M., Schulz, Richard, Talvani, Andre
Chemokines (CKs) and chemokine receptors (CKR) promote leukocyte recruitment into cardiac tissue infected by the Trypanosoma cruzi. This study investigated the long-term treatment with subantimicrobial doses of doxycycline (Dox) in association, or not, with benznidazole (Bz) on the expression of...
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Matrix metalloproteinase inhibition attenuates right ventricular dysfunction and improves responses to dobutamine during acute pulmonary thromboembolism
Download2013-01-01
Neto‐Neves, Evandro M., Sousa‐Santos, Ozelia, Ferraz, Karina C., Rizzi, Elen, Ceron, Carla S., Romano, Minna M., Schulz, Richard, Tanus‐Santos, Jose E.
Activated matrix metalloproteinases (MMPs) cause cardiomyocyte injury during acute pulmonary thromboembolism (APT). However, the functional consequences of this alteration are not known. We examined whether doxycycline (a MMP inhibitor) improves right ventricle function and the cardiac responses...
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Matrix metalloproteinase‐2, caveolins, focal adhesion kinase and c‐Kit in cells of the mouse myocardium
Download2007-01-01
Cho, Woo Jung, Chow, Ava K., Schulz, Richard, Daniel, Edwin E.
Matrix metalloproteinase‐2 (MMP‐2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav‐1, ‐2 and ‐3) are lipid raft proteins which play roles in cell sig‐nalling. This study examined, using immunohistochemistry and two photon...
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Multicentric osteolysis with nodulosis, arthritis, and cardiac defect syndrome: loss of MMP2 leads to increased apoptosis with alteration of apoptotic regulators and caspases and embryonic lethality
Download2014-01-01
Martignetti, John A., Mosig, Rebecca A., Schulz, Richard, Kassiri, Zamaneh, Schaffler, Mitchell B.
Inactivating mutations of matrix metalloproteinase 2 (MMP2) cause multicentric osteolysis with nodulosis and arthritis, one of a group of inherited osteolytic and arthritic disorders. We have previously shown that mice lacking Mmp2 share similar syndromic features with the human disorder, and at...
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Phosphorylation status of 72 kDa MMP-2 determines its structure and activity in response to peroxynitrite
Download2013-01-01
Jacob-Ferreira, Anna Laura, Kondo, Marcia Yuri, Baral, Pravas Kumar, James, Michael N., Holt, Andrew, Fan, Xiaohu, Schulz, Richard
Matrix metalloproteinase-2 (MMP-2) is a key intra- and extra-cellular protease which contributes to several oxidative stress related pathologies. A molecular understanding of 72 kDa MMP-2 activity, directly mediated by S-glutathiolation of its cysteine residues in the presence of peroxynitrite...
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2009-01-01
Joffe, Ari R., Schulz, Christina, Rosychuk, Ronda J., Dyck, John, Rebeyka, Ivan M., Ross, David B., Cheung, Po-Yin, Schulz, Richard
During cardiopulmonary-bypass matrix-metalloproteinases released may contribute to ventricular dysfunction. This study was to determine plasma matrix-metalloproteinases in neonates after cardiopulmonary-bypass and their relation to post-operative course. A prospective observational study included...
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Remodeling of aorta extracellular matrix as a result of transient high oxygen exposure in newborn rats: implication for arterial rigidity and hypertension risk
Download2014-01-01
Huyard, Fanny, Yzydorczyk, Catherine, Castro, Michele M., Cloutier, Anik, Bertagnolli, Mariane, Sartelet, Hervé, Schulz, Richard, Nuyt, Anne Monique
Neonatal high-oxygen exposure leads to elevated blood pressure, microvascular rarefaction, vascular dysfunction and arterial (aorta) rigidity in adult rats. Whether structural changes are present in the matrix of aorta wall is unknown. Considering that elastin synthesis peaks in late fetal life...
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Sequential fractionation and isolation of subcellular proteins from tissue or cultured cells
Download2015-01-01
Baghirova, Sabina, Hughes, Bryan G., Hendzel, Michael J., Schulz, Richard
Many types of studies require the localization of a protein to, or isolation of enriched protein from a specific cellular compartment. Many protocols in the literature and from commercially available kits claim to yield pure cellular fractions. However, in our hands, the former often do not work...
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Smooth muscle NOS, colocalized with caveolin‐1, modulates contraction in mouse small intestine
Download2008-01-01
Daniel, Edwin E., El‐Yazbi, Ahmed F., Cho, Woo Jung, Cena, Jonathan, Schulz, Richard
Neuronal nitric oxide synthase (nNOS) in myenteric neurons is activated during peristalsis to produce nitric oxide which relaxes intestinal smooth muscle. A putative nNOS is also found in the membrane of intestinal smooth muscle cells in mouse and dog. In this study we studied the possible...