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Stereo-selective deuteration in aspartate, asparagine, lysine, and methionine amino acid residues using fumarate as a carbon source for E. coli in D2O
Download2017-01-01
Danmaliki, Gaddafi I., Liu, Philip B., Hwang, Peter M.
We propose an isotope labeling strategy for expressing proteins in E. coli using protonated natural abundance 12C carbon sources in D2O. The strategy eliminates dominant 1H-13C and 1H-1H dipolar relaxation mechanisms to produce long-lived magnetization for solution NMR spectroscopy. Isolated 1H...
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