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Skip to Search Results- 16Electrospray ionization mass spectrometry
- 11Complexes
- 8Affinity
- 8Carbohydrates
- 7Affinities
- 6Binding
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2016
Han, Ling, Klassen, John S., Kitova, Elena N.
This study reports on the use of the catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) assay, combined with glycomicelles, as a method for detecting specific interactions between water-soluble proteins and glycolipids (GLs) in aqueous solution. The B subunit homopentamers...
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Dissociation kinetics of the streptavidin-biotin interaction measured using direct electrospray ionization mass spectrometry analysis
Download2013
Kitova, Elena N., Klassen, John S., Deng, Lu
Dissociation rate constants (koff) for the model high affinity interaction between biotin (B) and the homotetramer of natural core streptavidin (S4) were measured at pH 7 and temperatures ranging from 15 to 45 °C using electrospray ionization mass spectrometry (ESI-MS). Two different approaches...
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Dissociation kinetics of the streptavidin-biotin interaction measured using direct electrospray ionization mass spectrometry analysis
Download2013
Deng, Lu, Klassen, John S., Kitova, Elena N.
Dissociation rate constants (koff) for the model high affinity interaction between biotin (B) and the homotetramer of natural core streptavidin (S4) were measured at pH 7 and temperatures ranging from 15 to 45 °C using electrospray ionization mass spectrometry (ESI-MS). Two different approaches...
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2012
Klassen, John S., Lin, Hong, Kitova, Elena N., Ng, Kenneth K., Johnson, M. A., Eugenio, Luiz
Electrospray ionization mass spectrometry (ESI-MS) measurements were performed under a variety of solution conditions on a highly acidic sub-fragment (B3C) of the C-terminal carbohydrate-binding repeat region of Clostridium difficile toxin B, and two mutants (B4A and B4B) containing fewer acidic...
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2012
Klassen, John S., Lin, Hong, Kitova, Elena N., Ng, Kenneth K., Johnson, M. A., Eugenio, Luiz
Electrospray ionization mass spectrometry (ESI-MS) measurements were performed under a variety of solution conditions on a highly acidic sub-fragment (B3C) of the C-terminal carbohydrate-binding repeat region of Clostridium difficile toxin B, and two mutants (B4A and B4B) containing fewer acidic...
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2014
Kitova, Elena N., Klassen, John S., Michelsen, Klaus, Liu, Lan, Schnier, Paul D., Baergen, Alyson
This work explores the energetics of intermolecular H-bonds inside a hydrophobic protein cavity. Kinetic measurements were performed on the gaseous deprotonated ions (at the −7 charge state) of complexes of bovine β-lactoglobulin (Lg) and three monohydroxylated analogs of palmitic acid (PA):...
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2014
Kitova, Elena N., Klassen, John S., Michelsen, Klaus, Liu, Lan, Schnier, Paul D., Baergen, Alyson
This work explores the energetics of intermolecular H-bonds inside a hydrophobic protein cavity. Kinetic measurements were performed on the gaseous deprotonated ions (at the −7 charge state) of complexes of bovine β-lactoglobulin (Lg) and three monohydroxylated analogs of palmitic acid (PA):...
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2014
Klassen, John S., Derda, Ratmir, Ng, Simon, Bailey, Justin, Baergen, Alyson, Liu, Lan, Jalili, Nobar
This paper reports on the first experimental study of the energies of noncovalent fluorine bonding in a protein-ligand complex in the absence of solvent. Arrhenius parameters were measured for the dissociation of gaseous deprotonated ions of complexes of bovine β-lactoglobulin (Lg), a model...
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2014
Klassen, John S., Ng, Simon, Liu, Lan, Bailey, Justin, Derda, Ratmir, Baergen, Alyson, Jalili, Nobar
This paper reports on the first experimental study of the energies of noncovalent fluorine bonding in a protein-ligand complex in the absence of solvent. Arrhenius parameters were measured for the dissociation of gaseous deprotonated ions of complexes of bovine β-lactoglobulin (Lg), a model...
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Identifying carbohydrate ligands of a norovirus P particle using a catch and release electrospray ionization mass spectrometry assay
Download2014
Jiang, Xi, Kitova, Elena N., Klassen, John S., Tan, Ming, Han, Ling
Noroviruses (NoVs), the major cause of epidemic acute gastroenteritis, recognize human histo-blood group antigens (HBGAs), which are present as free oligosaccharides in bodily fluid or glycolipids and glycoproteins on the surfaces of cells. The subviral P particle formed by the protruding (P)...