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Skip to Search Results- 10Gänzle, Michael G.
- 4Garcia-Hernandez, Rigoberto
- 3McMullen, Lynn M.
- 2Li, Hui
- 2Mercer, Ryan G.
- 2Yang, Xianqin
- 10Agricultural, Food and Nutritional Science, Department of
- 10Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 1Graduate and Postdoctoral Studies (GPS), Faculty of
- 1Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
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Comparative assessment of qPCR enumeration methods that discriminate between live and dead Escherichia coli O157:H7 on beef
Download2018-01-01
Laidlaw, Anna M., Gänzle, Michael G., Yang, Xianqin
Quantitative Polymerase Chain Reaction (qPCR) is a molecular method commonly used to detect and quantify bacterial DNA on food but is limited by its inability to distinguish between live and dead cell DNA. To overcome this obstacle, propidium monoazide (PMA) alone or with deoxycholate (DC) was...
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Development and validation of a surrogate strain cocktail to evaluate bactericidal effects of pressure on verotoxigenic Escherichia coli
Download2015-01-01
Garcia-Hernandez, Rigoberto, McMullen, Lynn, Gänzle, Michael G.
Many strains of verotoxigenic Escherichia coli (VTEC) are highly resistant to pressure. To facilitate future studies to improve the elimination of VTEC by pressure processing of food, this study developed and validated a cocktail of non-pathogenic strains of E. coli with equal or higher...
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Effect of chitosan, and bacteriocin – Producing Carnobacterium maltaromaticum on survival of Escherichia coli and Salmonella Typhimurium on beef
Download2018-01-01
Hu, Zi Yi, Balay, Danielle, Hu, Ying, McMullen, Lynn M., Gänzle, Michael G.
The aim of this study was to investigate the synergistic effect of chitosan and bacteriocins against Escherichia coli and Salmonella in media and in lean beef. The inhibitory effects of chitosan and bacteriocins against E. coli AW1.7 and S. enterica Typhimurium in media were determined by a...
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Effect of hydrostatic pressure and antimicrobial on survival of Listeria monocytogenes and enterohaemorrhagic E.coli in beef
Download2016-01-01
The effects of marinades on Listeria monocytogenes and enterohaemorrhagic Escherichia coli were investigated in pressure treated beef steaks. Meat was treated with 600 MPa or 450 MPa. Marinades did not enhance pressure inactivation of E. coli in beef steaks and marinades prevented...
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2016-01-01
Li, Hui, Garcia-Hernandez, Rigoberto, Driedger, Darcy, McMullen, Lynn M., Gänzle, Michael G.
The pressure resistance of Shiga-toxin producing Escherichia coli (STEC) depends on food matrix. This study compared the resistance of two five-strain E. coli cocktails, as well as the pressure resistant strain E. coli AW1.7, to hydrostatic pressure application in bruschetta, tzatziki, yoghurt...
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2015
Garcia-Hernandez, Rigoberto, Ruan, Lifang, Gänzle, Michael G., Zheng, Jinshui, Mercer, Ryan G., McMullen, Lynn M.
Escherichia coli AW1.7 is a heat resistant food isolate and the occurrence of pathogenic strains with comparable heat resistance may pose a risk to food safety. To identify the genetic determinants of heat resistance, 29 strains of E. coli that differed in their of heat resistance were analyzed...
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Influence of cyclopropane fatty acids on heat, high pressure, acid, and oxidative stress resistance in Escherichia coli
Download2016-01-01
Chen, Yuan Yao, Gänzle, Michael G.
Heat and high pressure resistant strains of Escherichia coli are a challenge to food safety. This study investigated effects of cyclopropane fatty acids (CFAs) on stress tolerance in the heat- and pressure-resistant strain E. coli AW1.7 and the sensitive strain E. coli MG1655. The role of CFAs...
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Mechanisms of pressure-mediated cell death and injury in Escherichia coli: From fundamentals to food applications
Download2015
High hydrostatic pressure is commercially applied to extend the shelf life of foods, and to improve food safety. Current applications operate at ambient temperature and 600 MPa or less. However, bacteria that may resist this pressure level include the pathogens Staphylococcus aureus and strains...
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Structure and function relationships of the binding of β- and ɑ-galactosylated oligosaccharides to K88 fimbriae of enterotoxigenic Escherichia coli
Download2018-01-01
Yan, Ya Lu, Gänzle, Michael G.
Chitosan-oligosaccharides (COS) are suitable acceptors for β-galactosidase, and galactosylated-COS prevent adhesion of enterotoxigenic Escherichia coli (ETEC) K88 to porcine erythrocytes. The structure and functional relationship of oligosaccharide binding to ETEC K88 fimbriae was investigated....
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The locus of heat resistance (LHR) mediates heat resistance in Salmonella enterica, Escherichia coli and Enterobacter cloacae
Download2017-06-01
Mercer, Ryan G., Walker, Brian D., Yang, Xianqin, McMullen, Lynn M., Gänzle, Michael G.
Enterobacteriaceae comprise food spoilage organisms as well as food-borne pathogens including Escherichia coli. Heat resistance in E. coli was attributed to a genomic island called the locus of heat resistance (LHR). This genomic island is also present in several other genera of...