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2016

Han, Ling, Klassen, John S., Kitova, Elena N.

This study reports on the use of the catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) assay, combined with glycomicelles, as a method for detecting specific interactions between water-soluble proteins and glycolipids (GLs) in aqueous solution. The B subunit homopentamers...

2013

Kitova, Elena N., Klassen, John S., Deng, Lu

Dissociation rate constants (koff) for the model high affinity interaction between biotin (B) and the homotetramer of natural core streptavidin (S4) were measured at pH 7 and temperatures ranging from 15 to 45 °C using electrospray ionization mass spectrometry (ESI-MS). Two different approaches...

2013

Deng, Lu, Klassen, John S., Kitova, Elena N.

Dissociation rate constants (koff) for the model high affinity interaction between biotin (B) and the homotetramer of natural core streptavidin (S4) were measured at pH 7 and temperatures ranging from 15 to 45 °C using electrospray ionization mass spectrometry (ESI-MS). Two different approaches...

2012

Klassen, John S., Lin, Hong, Kitova, Elena N., Ng, Kenneth K., Johnson, M. A., Eugenio, Luiz

Electrospray ionization mass spectrometry (ESI-MS) measurements were performed under a variety of solution conditions on a highly acidic sub-fragment (B3C) of the C-terminal carbohydrate-binding repeat region of Clostridium difficile toxin B, and two mutants (B4A and B4B) containing fewer acidic...

2012

Klassen, John S., Lin, Hong, Kitova, Elena N., Ng, Kenneth K., Johnson, M. A., Eugenio, Luiz

Electrospray ionization mass spectrometry (ESI-MS) measurements were performed under a variety of solution conditions on a highly acidic sub-fragment (B3C) of the C-terminal carbohydrate-binding repeat region of Clostridium difficile toxin B, and two mutants (B4A and B4B) containing fewer acidic...

2014

Kitova, Elena N., Klassen, John S., Michelsen, Klaus, Liu, Lan, Schnier, Paul D., Baergen, Alyson

This work explores the energetics of intermolecular H-bonds inside a hydrophobic protein cavity. Kinetic measurements were performed on the gaseous deprotonated ions (at the −7 charge state) of complexes of bovine β-lactoglobulin (Lg) and three monohydroxylated analogs of palmitic acid (PA):...

2014

Kitova, Elena N., Klassen, John S., Michelsen, Klaus, Liu, Lan, Schnier, Paul D., Baergen, Alyson

This work explores the energetics of intermolecular H-bonds inside a hydrophobic protein cavity. Kinetic measurements were performed on the gaseous deprotonated ions (at the −7 charge state) of complexes of bovine β-lactoglobulin (Lg) and three monohydroxylated analogs of palmitic acid (PA):...

2014

Jiang, Xi, Kitova, Elena N., Klassen, John S., Tan, Ming, Han, Ling

Noroviruses (NoVs), the major cause of epidemic acute gastroenteritis, recognize human histo-blood group antigens (HBGAs), which are present as free oligosaccharides in bodily fluid or glycolipids and glycoproteins on the surfaces of cells. The subviral P particle formed by the protruding (P)...

2014

Jiang, Xi, Kitova, Elena N., Klassen, John S., Tan, Ming, Han, Ling

Noroviruses (NoVs), the major cause of epidemic acute gastroenteritis, recognize human histo-blood group antigens (HBGAs), which are present as free oligosaccharides in bodily fluid or glycolipids and glycoproteins on the surfaces of cells. The subviral P particle formed by the protruding (P)...

2009

Sun, Nian, Klassen, John S., Kitova, Elena N., Sun, Jiangxiao

The application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes...