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Skip to Search Results- 1Danmaliki, Gaddafi I.
- 1Feldman, Mario F.
- 1Fentabil, Messele A.
- 1Hug, Isabelle
- 1Hwang, Peter M.
- 1Klassen, John S.
- 1Bacterial Glycoproteins
- 1Bacterial amino acid biosynthetic pathways
- 1Central metabolic pathways
- 1Fucosyltransferase
- 1Glycoconjugate
- 1Glycoengineering
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Exploiting bacterial glycosylation machineries for the synthesis of a Lewis antigen-containing glycoprotein
Download2011
Hug, Isabelle, Reiz, Bela, Fentabil, Messele A., Klassen, John S., Whittal, Randy M., Zheng, Blake, Feldman, Mario F.
Glycoproteins constitute a class of compounds of increasing importance for pharmaceutical applications. The manipulation of bacterial protein glycosylation systems from Gram-negative bacteria for the synthesis of recombinant glycoproteins is a promising alternative to the current production...
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Stereo-selective deuteration in aspartate, asparagine, lysine, and methionine amino acid residues using fumarate as a carbon source for E. coli in D2O
Download2017-01-01
Danmaliki, Gaddafi I., Liu, Philip B., Hwang, Peter M.
We propose an isotope labeling strategy for expressing proteins in E. coli using protonated natural abundance 12C carbon sources in D2O. The strategy eliminates dominant 1H-13C and 1H-1H dipolar relaxation mechanisms to produce long-lived magnetization for solution NMR spectroscopy. Isolated 1H...