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Fall 2016
). Using protein engineering and directed evolution, we engineered FlicR1 to have sufficient speed and sensitivity to report single action potentials in single-trial recordings. Because it is excitable with yellow light, FlicR1 is the first FP-based voltage indicator that can be used in conjunction with
Fluorescence microscopy is currently the most powerful imaging technique for interrogation of neural circuits. Accordingly, tremendous efforts have been invested in engineering fluorescent proteins (FPs) to act as indicators for various biochemical processes in neural circuits, resulting in a
plethora of genetically encoded FP-based indicators for neural imaging. In this thesis, we report the development of various FP-based indicators for neuronal imaging. Specifically, we describe our efforts to expand the current repertoire of neuronal activity indicators to include a voltage indicator with