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- 8Mass-spectrometry
- 6Chemical isotope labeling
- 6LC-MS
- 3Metabolite identification
- 3Proteomics
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Development of Chemical Isotope Labeling (CIL) LC-MS Techniques for Untargeted Analysis Milk Metabolomics and Targeted Analysis of Propionic Acid
DownloadFall 2017
Metabolomics is the study of all the small molecules in a given sample or organism. It is used to understand individual variations caused by the relationship between the individual’s genetics, exposome and metabolic activity. There is interest in using metabolomics for personalized care leading...
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Development of Chemical Isotope Labeling Liquid Chromatography Mass Spectrometry for Cellular Metabolomics
DownloadFall 2018
Cellular metabolomics is the global study of a whole set of metabolites and its chemical processes in cells. Different analytical platforms, such as NMR and MS, have been employed for profiling the highly complex cellular metabolome and various data processing approaches have been developed. ...
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Development of Chemical Isotope Labeling Liquid Chromatography Mass Spectrometry on Quadrupole-Orbitrap Mass Spectrometers for Metabolome Analysis
DownloadFall 2023
Chemical isotope labeling liquid chromatography-mass spectrometry (CIL LC-MS) is a powerful technique in metabolomics, offering extensive coverage of the metabolome. This method involves dividing metabolites into sub-groups based on their functional groups and subjecting them to specific labeling...
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Development of Data Processing Methods in Chemical Isotope Labeling Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadSpring 2019
Metabolomics is an active research field on the methods development for the analysis of small metabolites in biological systems. It provides powerful approaches that allow us to examine the variations in the metabolic profiles and is capable of detecting complex biological changes using...
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Development of Liquid Chromatography Mass Spectrometry Methods for the Identification and Quantification of Acylcarnitines in Biological Samples
DownloadFall 2012
The field of metabolomics follows the Greek premise where metabolic changes are believed to be indicative of disease. Acylcarnitines, for example, can be dysregulated in the presence of various diseases including genetic metabolic disorders and multiple sclerosis. Liquid chromatography mass...
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Development of Mass Spectrometric Methods for Membrane Proteome Analysis and Protein Sequence Mapping
DownloadSpring 2014
This thesis work mainly focused on the development of mass spectrometric methods for membrane proteome analysis and protein sequence mapping. First, the comparison of the performance of RapiGest-, PPS- and SDS-based sample preparation methods for shotgun membrane proteome analysis was...
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Fall 2017
The most often used biofluids in metabolomics and disease biomarker discovery studies in the literature include urine, processed blood (serum or plasma), and cerebral spinal fluid. While investigations using these biofluids have led to numerous innovations in the field of metabolomics, little...
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Differential 15N2-/14N2-isotope Dansylhydrazine Labeling and LC-MS for Quantification of the Human Carbonyl Metabolome
DownloadFall 2011
The objective of this work was the design and use of paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust means of quantitative mass spectrometry (MS) based metabolome profiling. Herein is presented the differential 15N2-/14N2-isotope...
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Evaluation of Derivatization Reaction Conditions for Metabolite Labelling Using Differential Isotopic Labelling for Biological Samples Analyzed by Liquid Chromatography Mass Spectrometry
DownloadFall 2013
The objective of this work was to assess the impact of reaction conditions for isotopic labeling of metabolites prior to analysis using liquid chromatography mass spectrometry (LC-MS). Differential isotope 12C/13C dansyl chloride was used to label metabolites containing either primary amines,...
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Isotope Labeling Liquid Chromatography Mass Spectrometry: Metabolite Identification and Human Salivary Metabolome Profiling
DownloadFall 2012
The objective of this work was to use paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust way of mass spectrometry (MS)-based metabolome profiling. A differential 13C-/12C-isotope dansyl chloride (DnsCl) derivatization strategy has been...