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Spring 2021
Splicing is an essential step in the post-transcriptional modification of pre-mRNAs in eukaryotes. This process is catalyzed by the spliceosome, a multi-megadalton nuclear protein-RNA enzyme which excises non-coding sequence from RNA transcripts prior to translation. Deficient or improper...
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Spring 2023
The discontinuous split-gene structure is a core feature of eukaryotic gene architecture and requires pre-mRNA splicing to remove silent intron sequences from the initial pre-mRNA transcript before it becomes a mature mRNA suitable for protein synthesis. Splicing begins with recognition of both...
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Spring 2019
Exons, protein coding regions in eukaryotic genes, are interrupted by non-coding introns that must be removed from transcribed pre-mRNA prior to translation into protein. Introns are removed, and exons ligated, in a process called pre-mRNA splicing. Conserved sites within the intron, the 5′ and...