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Skip to Search Results- 33Mass-spectrometry
- 8Metabolomics
- 6Proteomics
- 3Electrospray ionization
- 3Isotope labeling
- 3Liquid chromatography
- 1Alexander, Anthony John.
- 1Chen, Lu
- 1Dawe, Margot Renee
- 1De Souza, Andrea
- 1Dunlop, Kevin Yoshio.
- 1Fentabil, Messele
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Development of Mass Spectrometric Methods for Membrane Proteome Analysis and Protein Sequence Mapping
DownloadSpring 2014
This thesis work mainly focused on the development of mass spectrometric methods for membrane proteome analysis and protein sequence mapping. First, the comparison of the performance of RapiGest-, PPS- and SDS-based sample preparation methods for shotgun membrane proteome analysis was...
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Fall 2016
This thesis describes the development of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was first applied to quantify protein-carbohydrate interactions in aqueous ammonium acetate...
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Spring 2010
Interest in microfluidic platforms has surged as an alternative for sample preparation in the past two decades, with the potential for miniaturization, portability, automation, integration and parallelism driving this research. However, it is still very challenging to develop an integrated...
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Differential 15N2-/14N2-isotope Dansylhydrazine Labeling and LC-MS for Quantification of the Human Carbonyl Metabolome
DownloadFall 2011
The objective of this work was the design and use of paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust means of quantitative mass spectrometry (MS) based metabolome profiling. Herein is presented the differential 15N2-/14N2-isotope...
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Investigating Protein-Carbohydrate Interactions with Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS)
DownloadFall 2014
The application of hydrogen/deuterium exchange mass spectrometry (HDX-MS) to investigating protein-carbohydrate interactions is described. Proteins from three bacterial toxins, the B subunit homopentamers of Cholera toxin (CTB5) and Shiga toxin type 1 (Stx1B5) and a fragment of Clostridium...
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Isotope Labeling Liquid Chromatography Mass Spectrometry: Metabolite Identification and Human Salivary Metabolome Profiling
DownloadFall 2012
The objective of this work was to use paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust way of mass spectrometry (MS)-based metabolome profiling. A differential 13C-/12C-isotope dansyl chloride (DnsCl) derivatization strategy has been...