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Skip to Search Results- 52Mass-spectrometry
- 8Metabolomics
- 6Cytotoxicity
- 5Electrospray ionization
- 4Classification
- 4Proteomics
- 6Klassen, John S.
- 4Kitova, Elena N.
- 3Han, Ling
- 2Armstrong, Glen D.
- 2Dingle, Tanis C.
- 2El-Hawiet, Amr
- 44Graduate and Postdoctoral Studies (GPS), Faculty of
- 44Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 7Chemistry, Department of
- 7Chemistry, Department of/Journal Articles (Chemistry)
- 2Biological Sciences, Department of
- 2Biological Sciences, Department of/Journal Articles (Biological Sciences)
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Fall 2016
This thesis describes the development of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was first applied to quantify protein-carbohydrate interactions in aqueous ammonium acetate...
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Differential 15N2-/14N2-isotope Dansylhydrazine Labeling and LC-MS for Quantification of the Human Carbonyl Metabolome
DownloadFall 2011
The objective of this work was the design and use of paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust means of quantitative mass spectrometry (MS) based metabolome profiling. Herein is presented the differential 15N2-/14N2-isotope...
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Fall 2013
High throughput bio-technologies in chemistry experiments generate a huge amount of data. These data are awaiting to be analyzed for knowledge discovery. In this work, we have developed a web-based resource MyCompoundID for compound identification. Our base database contains 8,021 metabolite...
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In-line Ozonolysis coupled to Mass Spectrometry- A New Dimension of Structural Determination for Lipids
DownloadFall 2014
The location of double bonds within unsaturated lipids can greatly affect their biological functions. Liquid chromatography coupled to mass spectrometry (LC/MS) has become a powerful tool for lipid separation and structural elucidation. However, the determination of double bond position remains...
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Investigating Protein-Carbohydrate Interactions with Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS)
DownloadFall 2014
The application of hydrogen/deuterium exchange mass spectrometry (HDX-MS) to investigating protein-carbohydrate interactions is described. Proteins from three bacterial toxins, the B subunit homopentamers of Cholera toxin (CTB5) and Shiga toxin type 1 (Stx1B5) and a fragment of Clostridium...
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Isotope Labeling Liquid Chromatography Mass Spectrometry: Metabolite Identification and Human Salivary Metabolome Profiling
DownloadFall 2012
The objective of this work was to use paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust way of mass spectrometry (MS)-based metabolome profiling. A differential 13C-/12C-isotope dansyl chloride (DnsCl) derivatization strategy has been...