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- 22Carbohydrates
- 4Affinity
- 4Electrospray ionization mass spectrometry
- 4Expressions
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- 28Graduate and Postdoctoral Studies (GPS), Faculty of
- 28Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 15Chemistry, Department of
- 15Chemistry, Department of/Journal Articles (Chemistry)
- 12Biological Sciences, Department of
- 12Biological Sciences, Department of/Journal Articles (Biological Sciences)
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2010
Sensen, C.W., Goss, G.G., Childs, S.J., Zuccolo, J., Bau, J., Deans, J.P.
Background: The MS4A gene family in humans includes CD20 (MS4A1), FcRb (MS4A2), Htm4 (MS4A3), and at least 13 other syntenic genes encoding membrane proteins, most having characteristic tetraspanning topology. Expression of MS4A genes is variable in tissues throughout the body; however, several...
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Probing microscopic conformational dynamics in folding reactions by measuring transition paths
Download2019-12-01
Hoffer, Noel Q, Woodside, Michael T
Transition paths comprise those parts of a folding trajectory where the molecule passes through the high-energy transition states separating folded and unfolded conformations. The transition states determine the folding kinetics and mechanism but are difficult to observe because of their brief...
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Proteome differences associated with fat accumulation in bovine subcutaneous adipose tissues
Download2010
Basu, U., Guan, L., Dodson, M. V., Zhao, Y. M., Basarab, J. A.
Background The fat components of red meat products have been of interest to researchers due to the health aspects of excess fat consumption by humans. We hypothesized that differences in protein expression have an impact on adipose tissue formation during beef cattle development and growth....
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Purification and characterization of an anaerobically induced alanine aminotransferase from barley roots
Download1992
Alanine aminotransferase (AlaAT, EC 2.6.1.2) is an enzyme that is induced under anaerobic conditions in cereal roots. In barley (Hordeum vulgare L.) roots, there are a number of isoforms of AlaAT. We have identified the anaerobically induced isoform and have purified it to homogeneity. The...
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Quantifying protein-carbohydrate interactions using liquid sample desorption electrospray ionization mass spectrometry
Download2015
N. Kitova, Elena N., Shams-Ud-Doha, Km, Yao, Yuyu, Klassen, John S., Daneshfar, Rambod
The application of liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) for quantifying protein–carbohydrate interactions in vitro is described. Association constants for the interactions between lysozyme and β-D-GlcNAc-(1 → 4)-β-D-GlcNAc-(1 → 4)-D-GlcNAc and...
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Quantifying protein-carbohydrate interactions using liquid sample desorption electrospray ionization mass spectrometry
Download2015
Klassen, John S., N. Kitova, Elena N., Yao, Yuyu, Shams-Ud-Doha, Km, Daneshfar, Rambod
The application of liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) for quantifying protein–carbohydrate interactions in vitro is described. Association constants for the interactions between lysozyme and β-D-GlcNAc-(1 → 4)-β-D-GlcNAc-(1 → 4)-D-GlcNAc and...
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Resource allocation, water relations and crown architecture examined at the tree and stand-level in northern conifers
DownloadFall 2013
Variation in quantity of light has driven plants to employ many strategies in order to persist in high and low light. It is also a primary driver of lower branch mortality and crown recession. Fine roots and leaves are complimentary tissues representing belowground and aboveground resource...
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Screening carbohydrate libraries for protein interactions using the direct ESI-MS assay: Applications to libraries of unknown concentration
Download2014
El-Hawiet, Amr, Klassen, John S., Kitova, Elena N.
A semiquantitative electrospray ionization mass spectrometry (ESI-MS) binding assay suitable for analyzing mixtures of oligosaccharides, at unknown concentrations, for interactions with target proteins is described. The assay relies on the differences in the ratio of the relative abundances of...