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Skip to Search Results- 3Keeling, Christopher I.
- 2Bohlmann, Jörg
- 2Bonnett, Tiffany R.
- 2Hamelin, Richard C.
- 2Pitt, Caitlin
- 2Robert, Jeanne A.
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The construction and use of bacterial DNA microarrays based on an optimized two-stage PCR strategy
Download2003
Li, H., Klahn, J., Galbraith, D.W., Deyholos, M., Sherman, L.A., Impson, L., Postier, B.L., Risinger, G., Andrews, H.L., Burnap, R.L., Pesta, D., Wang, H.L., Singh, A.
Background: DNA microarrays are a powerful tool with important applications such as global gene expression profiling. Construction of bacterial DNA microarrays from genomic sequence data using a two-stage PCR amplification approach for the production of arrayed DNA is attractive because it...
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Spatiotemporal integration of molecular and anatomical data in virtual reality using semantic mapping
Download2009
Janzen, R.P., Dong, X., Gordon, P.M., Soh, J., Wishart, D.S., Sabhaney, A., Turinsky, A.L., Sensen, C.W., Chang, J., Hau, D., Xia, J., Trinh, Q.M.
We have developed a computational framework for spatiotemporal integration of molecular and anatomical datasets in a virtual reality environment. Using two case studies involving gene expression data and pharmacokinetic data, respectively, we demonstrate how existing knowledge bases for molecular...
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Spatial genetic structure of a symbiotic beetle-fungal system: Toward multi-taxa integrated landscape genetics
Download2011-01-01
Sperling, Felix A. H., Hamelin, Richard C., Murray, Brent W., James, Patrick M. A., Coltman, Dave W.
Spatial patterns of genetic variation in interacting species can identify shared features that are important to gene flow and can elucidate co-evolutionary relationships. We assessed concordance in spatial genetic variation between the mountain pine beetle (Dendroctonus ponderosae) and one of its...
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Shape Variation of Cysticercoids of Hymenolepis diminuta (Cyclophyllidea) from Fed, Partially Fed, and Fasted Tribolium confusum (Coleoptera)
Download2006
Quantitative studies of a crowding effect on cysticercoids of Hymenolepis diminuta in the intermediate host are few and limited in scope. In this study, we developed a technique to rapidly collect morphological information on large numbers of parasites, and verified the utility of geometric...
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Proteomics indicators of the rapidly shifting physiology from whole mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae), adults during early host colonization
Download2014-01-01
Bohlmann, Jörg, Pitt, Caitlin, Huber, Dezene P. W. , Bonnett, Tiffany R., Keeling, Christopher I., Robert, Jeanne A.
We developed proteome profiles for host colonizing mountain pine beetle adults, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae). Adult insects were fed in pairs on fresh host lodgepole pine, Pinus contorta Dougl. ex Loud, phloem tissue. The proteomes of fed individuals were monitored...
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Population Structure of Mountain Pine Beetle Symbiont Leptographium longiclavatum and the Implication on the Multipartite Beetle-Fungi Relationships
Download2014-01-01
Roe, Amanda D., Farfan, Lina, Cooke, Janice E. K., Hamelin, Richard C., El-Kassaby, Yousry A., Rice, Adrianne V., Tsui, Clement K.
Over 18 million ha of forests have been destroyed in the past decade in Canada by the mountain pine beetle (MPB) and its fungal symbionts. Understanding their population dynamics is critical to improving modeling of beetle epidemics and providing potential clues to predict population expansion....
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Molecular cloning, pharmacological characterization and brain mapping of the melanocortin 4 receptor in the goldfish: Involvement in the control of food intake
Download2003
Schioth, H.B., Cerdá-Reverter, J.M., Peter, R.E., Ringholm, A.
We report cloning, pharmacological characterization, tissue distribution, detailed brain mapping, and role in control of food intake of melanocortin 4 receptor in goldfish (gMC4R). The gMC4R protein has 68% identity with the human ortholog and is conserved in important functional domains....
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Generation, annotation, analysis and database integration of 16,500 white spruce EST clusters
Download2005
Johnson, J.E., Pavy, N., Cooke, J., Parsons, L., Paule, C., Morency, M.J., Crow, J.A.
Background: The sequencing and analysis of ESTs is for now the only practical approach for largescale gene discovery and annotation in conifers because their very large genomes are unlikely to be sequenced in the near future. Our objective was to produce extensive collections of ESTs and cDNA...
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Gawky Is a Component of Cytoplasmic mRNA Processing Bodies Required for Early Drosophila Development
Download2006
Hobman, T. C., Locker, J., Schneider, M. D., Pare, J. M., Haskins, J., Simmonds, A. J., Chaker, S., Najand, N
In mammalian cells, the GW182 protein localizes to cytoplasmic bodies implicated in the regulation of messenger RNA ( mRNA) stability, translation, and the RNA interference pathway. Many of these functions have also been assigned to analogous yeast cytoplasmic mRNA processing bodies. We have...