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- 1Chemical cleavage
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- 1Fusion protein expression
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- 1Intrinsically disordered regions
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Combining a PagP fusion protein system with nickel ion-catalyzed cleavage to produce intrinsically disordered proteins in E. coli
Download2015-01-01
Zahran, Somaya, Pan, Jonathan S., Liu, Philip B., Hwang, Peter M.
Many proteins contain intrinsically disordered regions that are highly solvent-exposed and susceptible to post-translational modifications. Studying these protein segments is critical to understanding their physiologic regulation, but proteolytic degradation can make them difficult to express and...
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