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Skip to Search Results- 1Abdelfattah, Ahmed
- 1Adam, Ibrahim S. Y.
- 1Akinwumi, Segun M
- 1Alford, Spencer Caleb
- 1Anbukarasu, Preetam
- 1Assempour, Nazanin
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Fall 2014
The discovery and subsequent applications of fluorescent proteins (FPs) launched a new era for live cell fluorescence imaging. The design and developments of FP-based indicators have further solidified the versatility of FPs and rendered them as indispensable tools in life science research now...
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Fall 2011
AC Dielectrophoresis, the motion of dielectric colloidal particles in the presence of an inhomogeneous AC electric field, has been utilized in this work as a mechanism for preventing channel wall fouling due to particle deposition for a multi-component colloidal system. Both attractive and...
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Directed Evolution Approaches for Improved Genetically Encoded Fluorescent Calcium Ion and Voltage Indicators
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Fluorescent proteins (FP) have revolutionized our imaging technologies and facilitated visualization of biochemical and physiological processes occurring in complex biological systems, which has opened up a new and unprecedented era in cell biology. Although FPs mainly serve as passive...
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Spring 2013
The sources of inducing field gradients and their implications are investigated inside the microfabricated post arrays. A parametric study is conducted to understand how the posts arrangements, distance, and size change the field and field gradients. The results provide criteria where the...
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Spring 2015
Fluorescent proteins (FP) and FP-based biosensors have become essential tools for cell biology and neuroscience research. This thesis describes efforts to engineer new FPs with red emission and novel genetically encoded biosensors based on red FPs. Directed evolution and semi-rational design are...
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Fall 2022
Affinity maturation is the process to improve antibody affinity for an antigen during an adaptive immune response; it is mediated by the immunoglobulin mutator enzyme activation-induced cytidine deaminase (AID). This process is crucial to produce high-affinity memory B-cells and plasma cells. In...
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Fall 2015
The expansion of the fluorescent protein (FP) colour palette through discovery of more species possessing FPs and by subjecting them to intensive protein engineering efforts has greatly aided the effectiveness of FP-based biosensor designs. Currently, the main FP-based methods for detecting...
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Expanding the optogenetic toolkit with a photocleavable protein and a near infrared pH indicator
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Optogenetics is an emerging biological technique that is causing a revolution in life science. It uses optical methods to control and visualize genetically targeted biological events in a relatively non-invasive and spatiotemporally precise manner. Optogenetics requires advanced optical...
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Spring 2013
Research was undertaken into the organic semiconductors tris(8- hydroxyquinoline)aluminum (Alq3) as well as bis(8-hydroxyquinoline)zinc (Znq2) to the effect of improving achievable morphological control using an inexpensive evaporation based solution synthesis method. By varying experimental...
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Fall 2022
Background: Although pediatric heart transplantation is a life-saving and effective therapy for children with cardiac disease, barriers remain due to a lack of available organs as well as transplant failure due to rejection. This dissertation aimed to increase knowledge related to immune risk...