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2014
Kitova, Elena N., Klassen, John S., Michelsen, Klaus, Liu, Lan, Schnier, Paul D., Baergen, Alyson
This work explores the energetics of intermolecular H-bonds inside a hydrophobic protein cavity. Kinetic measurements were performed on the gaseous deprotonated ions (at the −7 charge state) of complexes of bovine β-lactoglobulin (Lg) and three monohydroxylated analogs of palmitic acid (PA):...
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2014
Kitova, Elena N., Klassen, John S., Michelsen, Klaus, Liu, Lan, Schnier, Paul D., Baergen, Alyson
This work explores the energetics of intermolecular H-bonds inside a hydrophobic protein cavity. Kinetic measurements were performed on the gaseous deprotonated ions (at the −7 charge state) of complexes of bovine β-lactoglobulin (Lg) and three monohydroxylated analogs of palmitic acid (PA):...
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Localizing carbohydrate binding sites in proteins using hydrogen/deuterium exchange mass spectrometry
Download2016
Kitova, Elena N., Klassen, John S., Eugenio, Luiz, Li, Jun, Ng, Kenneth, Zhang, Jingjing
The application of hydrogen/deuterium exchange mass spectrometry (HDX-MS) to localize ligand binding sites in carbohydrate-binding proteins is described. Proteins from three bacterial toxins, the B subunit homopentamers of Cholera toxin and Shiga toxin type 1 and a fragment of Clostridium...
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Localizing carbohydrate binding sites in proteins using hydrogen/deuterium exchange mass spectrometry
Download2016
Klassen, John S., Kitova, Elena N., Li, Jun, Zhang, Jingjing, Ng, Kenneth, Eugenio, Luiz
The application of hydrogen/deuterium exchange mass spectrometry (HDX-MS) to localize ligand binding sites in carbohydrate-binding proteins is described. Proteins from three bacterial toxins, the B subunit homopentamers of Cholera toxin and Shiga toxin type 1 and a fragment of Clostridium...