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Skip to Search Results- 7Hamelin, Richard C.
- 6Breuil, Colette
- 5Bohlmann, Jörg
- 4DiGuistini, Scott
- 4Robertson, Gordon
- 3Cooke, Janice E. K.
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A specialized ABC efflux transporter GcABC-G1 confers monoterpene resistance to Grosmannia clavigera, a bark beetle-associated fungal pathogen of pine trees
Download2013-01-01
Bohlmann, Jörg, Wang, Ye, Breuil, Colette, DiGuistini, Scott, Lim, Lynette, Robertson, Gordon
Grosmannia clavigera is a bark beetle-vectored pine pathogen in the mountain pine beetle epidemic in western North America. Grosmannia clavigera colonizes pines despite the trees' massive oleoresin terpenoid defences. We are using a functional genomics approach to identify G. clavigera's...
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2014-01-01
Alamouti, Sepideh Massoumi, Haridas, Sajeet, Feau, Nicolas, Robertson, Gordon, Bohlmann, Jorg, Breuil, Colette
Studies on beetle/tree fungal symbionts typically characterize the ecological and geographic distributions of the fungal populations. There is limited understanding of the genome-wide evolutionary processes that act within and between species as such fungi adapt to different environments, leading...
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De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data
Download2009-01-01
Docking, Roderick T., Mardis, Elaine, Hamelin, Richard C., Jones, Steven J. M., Marra, Marco A., Seidel, Michael, Chan, Simon K., Breuil, Colette, Liao, Nancy Y., Birol, Inanc, Robertson, Gordon, Holt, Robert A., Platt, Darren, Bohlmann, Jörg, DiGuistini, Scott, Hirst, Martin
Sequencing-by-synthesis technologies can reduce the cost of generating de novo genome assemblies. We report a method for assembling draft genome sequences of eukaryotic organisms that integrates sequence information from different sources, and demonstrate its effectiveness by assembling an...
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Fine-scale genetic diversity and relatedness in fungi associated with the mountain pine beetle
Download2019-01-01
Tsui, Clement K. M., Beauseigle, Stephanie, Ojeda Alayon, Dario I., Rice, Adrianne V., Cooke, Janice E. K., Sperling, Felix A. H., Roe, Amanda D., Hamelin, Richard C.
The mountain pine beetle (MPB; Dendroctonus ponderosae Hopkins, 1902) forms beneficial symbiotic associations with fungi. Here we explored the fine-scale spatial genetic structure of three of those fungi using single nucleotide polymorphism. We found that single mated pairs of beetles carry not...
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Gene discovery for enzymes involved in limonene modification or utilization by the mountain pine beetle-associated pathogen Grosmannia clavigera
Download2014-01-01
Lah, Ljerka, Wang, Ye, Breuil, Colette, Madilao, Lina, Bohlmann, Joerg, Lim, Lynette
To successfully colonize and eventually kill pine trees, Grosmannia clavigera (Gs cryptic species), the main fungal pathogen associated with the mountain pine beetle (Dendroctonus ponderosae), has developed multiple mechanisms to overcome host tree chemical defenses, of which terpenoids are a...
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2010-01-01
Henderson, Hannah, Keeling, Christopher I.Wang, Ye, Docking, Roderick T., Breuil, Colette, Li, Maria, Jones, Steven J. M., Liao, Nancy Y., Hesse-Orce, Uljana, DiGuistini, Scott, Robertson, Gordon, Bohlmann, Jörg, Holt, Robert A.
Background Grosmannia clavigera is a bark beetle-vectored fungal pathogen of pines that causes wood discoloration and may kill trees by disrupting nutrient and water transport. Trees respond to attacks from beetles and associated fungi by releasing terpenoid and phenolic defense compounds. It is...
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Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera
Download2020-02-01
Wong, Barbara, Leal, Isabel, Feau, Nicolas, Dale, Angela, Uzunovic, Adnan, Hamelin, Richard C.
Wood and wood products can harbor microorganisms that can raise phytosanitary concerns in countries importing or exporting these products. To evaluate the efficacy of wood treatment on the survival of microorganisms of phytosanitary concern the method of choice is to grow microbes in petri dishes...
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Population Structure of Mountain Pine Beetle Symbiont Leptographium longiclavatum and the Implication on the Multipartite Beetle-Fungi Relationships
Download2014-01-01
Roe, Amanda D., Farfan, Lina, Cooke, Janice E. K., Hamelin, Richard C., El-Kassaby, Yousry A., Rice, Adrianne V., Tsui, Clement K.
Over 18 million ha of forests have been destroyed in the past decade in Canada by the mountain pine beetle (MPB) and its fungal symbionts. Understanding their population dynamics is critical to improving modeling of beetle epidemics and providing potential clues to predict population expansion....
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Spatial genetic structure of a symbiotic beetle-fungal system: Toward multi-taxa integrated landscape genetics
Download2011-01-01
Sperling, Felix A. H., Hamelin, Richard C., Murray, Brent W., James, Patrick M. A., Coltman, Dave W.
Spatial patterns of genetic variation in interacting species can identify shared features that are important to gene flow and can elucidate co-evolutionary relationships. We assessed concordance in spatial genetic variation between the mountain pine beetle (Dendroctonus ponderosae) and one of its...
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The contribution of genetics and genomics to understanding the ecology of the mountain pine beetle system
Download2019-01-01
Cullingham, Catherine I., Janes, Jasmine K., Hamelin, Richard C., James, Patrick M.A., Murray, Brent W., Sperling, Felix A.H.
Environmental change is altering forest insect dynamics worldwide. As these systems change, they pose significant ecological, social, and economic risk through, for example, the loss of valuable habitat, green space, and timber. Our understanding of such systems is often limited by the complexity...