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Skip to Search Results- 13Metabolomics
- 8Mass-spectrometry
- 6Chemical isotope labeling
- 6LC-MS
- 3Metabolite identification
- 3Proteomics
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Differential 15N2-/14N2-isotope Dansylhydrazine Labeling and LC-MS for Quantification of the Human Carbonyl Metabolome
DownloadFall 2011
The objective of this work was the design and use of paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust means of quantitative mass spectrometry (MS) based metabolome profiling. Herein is presented the differential 15N2-/14N2-isotope...
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Development and Applications of Stable Isotope Labelling Liquid Chromatography Mass Spectrometry for Quantitative Proteomics
DownloadSpring 2011
This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...
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Development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts
DownloadSpring 2011
An enantioselective heart-cut two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method was developed for the analysis of 25 methylsulfonyl polychlorinated biphenyl metabolites in tissue extracts. Enantioseparation was achieved for 9 out of the 10...
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Isotope Labeling Liquid Chromatography Mass Spectrometry: Metabolite Identification and Human Salivary Metabolome Profiling
DownloadFall 2012
The objective of this work was to use paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust way of mass spectrometry (MS)-based metabolome profiling. A differential 13C-/12C-isotope dansyl chloride (DnsCl) derivatization strategy has been...
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Metabolite Identification and Quantification in Biofluids Using Liquid Chromatography Mass Spectrometry
DownloadFall 2012
Metabolomics, one of the branches of systems biology, is the comprehensive measurement of all the endogenous metabolites in a biological system. It can be applied in the areas of biomarker discovery and diagnosis of critical diseases and disorders, and can significantly increase our understanding...
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Development of Liquid Chromatography Mass Spectrometry Methods for the Identification and Quantification of Acylcarnitines in Biological Samples
DownloadFall 2012
The field of metabolomics follows the Greek premise where metabolic changes are believed to be indicative of disease. Acylcarnitines, for example, can be dysregulated in the presence of various diseases including genetic metabolic disorders and multiple sclerosis. Liquid chromatography mass...
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Fall 2012
The overall goal of this thesis research is to develop a spectral searching strategy capable of identifying peptide sequences from MS/MS spectra with high sensitivity and accuracy. First, a shotgun proteome analysis method was developed and successfully applied to the identification of proteins...
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Evaluation of Derivatization Reaction Conditions for Metabolite Labelling Using Differential Isotopic Labelling for Biological Samples Analyzed by Liquid Chromatography Mass Spectrometry
DownloadFall 2013
The objective of this work was to assess the impact of reaction conditions for isotopic labeling of metabolites prior to analysis using liquid chromatography mass spectrometry (LC-MS). Differential isotope 12C/13C dansyl chloride was used to label metabolites containing either primary amines,...
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Development and Applications of Mass Spectrometric Methods for Proteome Analysis and Protein Sequence Characterization
DownloadFall 2013
Mass spectrometry (MS) is widely used in proteomic work for protein identification and characterization. A key to the success of MS for protein analysis is related to sample preparation. Current sample preparation methods do not meet all the needs of protein or proteome analysis. My thesis work...
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Development of Mass Spectrometric Methods for Membrane Proteome Analysis and Protein Sequence Mapping
DownloadSpring 2014
This thesis work mainly focused on the development of mass spectrometric methods for membrane proteome analysis and protein sequence mapping. First, the comparison of the performance of RapiGest-, PPS- and SDS-based sample preparation methods for shotgun membrane proteome analysis was...