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- 1Arabidopsis thaliana
- 1Genetically encoded biosensor
- 1LACS
- 1Saccharomyces cerevisiae
- 1error-prone PCR
- 1Caldo, Kristian Mark P.
- 1Chen, Guanqun
- 1Holic, Roman
- 1Mietkiewska, Elzbieta
- 1Ozga, Jocelyn
- 1Rizvi, Syed Masood
- 1Agricultural, Food and Nutritional Science, Department of
- 1Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 1Graduate and Postdoctoral Studies (GPS), Faculty of
- 1Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
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Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity
Download2018-12-17
Xu, Yang, Caldo, Kristian Mark P., Holic, Roman, Mietkiewska, Elzbieta, Ozga, Jocelyn, Rizvi, Syed Masood, Chen, Guanqun, Weselake, Randall
Long-chain acyl-CoA synthetase (LACS, EC 6.2.1.3) catalyzes the ATP-dependent activation of free fatty acid to form acyl-CoA, which in turn serves as the major acyl donor for various lipid metabolic pathways. Increasing the size of acyl-CoA pool by enhancing LACS activity appears to be a useful...
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Genetically encoded fluorescent biosensors for detection of ligands and protein-protein interactions
DownloadSpring 2021
Fluorescence imaging enables direct visualization of cellular activities with remarkable resolutions supported by advancing technologies. The fluorescent markers for biomolecules complement the hardware and software for imaging a specific target in detail. Genetically encoded fluorescent proteins...