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Fall 2016
Two-photon absorption (TPA) microscopy of fluorescent proteins (FPs) is a powerful bio-imaging tool. Advantages of TPA microscopy include better focus and less out-of-focus bleaching, together with absorption at longer wavelengths than in one-photon absorption (OPA), which leads to deeper...
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One-, Two-, and Three-Photon Absorption Studies of Fluorescent Proteins and Their Chromophores Using Quantum Mechanical and Quantum Mechanical/Molecular Mechanical Approaches
DownloadFall 2021
Rossano Tapia, Maria del Sagrario
In the present thesis, the multi-photon absorption features of different Aequorea fluorescent proteins (FPs) are explored computationally using quantum mechanical and combined quantum mechanical/molecular mechanical approaches. In chapters two and three of the present thesis, we evaluate the...