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2008
Background: Bast fibres from the phloem tissues of flax are scientifically interesting and economically useful due in part to a dynamic system of secondary cell wall deposition. To better understand the molecular mechanisms underlying the process of cell wall development in flax, we extracted...
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Differential exudation of polypeptides by roots of aluminum-resistant and aluminum-sensitive cultivars of Triticum aestivum L. in response to aluminum stress
Download1994
Basu, A., Taylor, G. J., Basu, U.
Cultivars of Triticum aestivum differing in resistance to Al were grown under aseptic conditions in the presence and absence of Al and polypeptides present in root exudates were collected, concentrated, and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Upon exposure to...
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Drosophila ATM and ATR have distinct activities in the regulation of meiotic DNA damage and repair
Download2011
Tiong, S., McKim, K.S., Campbell, S.D., White-Brown, S.K., Paul, A., Pedersen, M., Joyce, E.F.
Ataxia telangiectasia–mutated (ATM) and ataxia telangiectasia–related (ATR) kinases are conserved regulators of cellular responses to double strand breaks (DSBs). During meiosis, however, the functions of these kinases in DSB repair and the deoxyribonucleic acid (DNA) damage checkpoint are...
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Endogenous melanocortin antagonist in fish: Structure, brain mapping and regulation by fasting of the goldfish agouti-related protein gene
Download2003
Cerdá-Reverter, J.M., Peter, R.E.
Agouti-related protein (AGRP) is a naturally occurring antagonist of melanocortin. In mammals, central AGRP expression is restricted to the arcuate nucleus in which it plays a key role in the control of energy balance by antagonizing melanocortin effects at melanocortin 4 receptors. In goldfish,...
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2001
Bohnert, H. J., Deyholos, M., Brazille, S., Wang, H., Kawai, K., Borchert, C., Galbraith, D., Kawasaki, S.
Transcript regulation in response to high salinity was investigated for salt-tolerant rice (var Pokkali) with microarrays including 1728 cDNAs from libraries of salt-stressed roots. NaCl at 150 mM reduced photosynthesis to one tenth of the prestress value within minutes. Hybridizations of RNA to...
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Generation of a restriction minus enteropathogenic Escherichia coli E2348/69 strain that is efficiently transformed with large, low copy plasmids
Download2008
Raivio, T.L., Price, N.L., Ward, J.D., Hobson, N.
Background Many microbes possess restriction-modification systems that protect them from parasitic DNA molecules. Unfortunately, the presence of a restriction-modification system in a given microbe also hampers genetic analysis. Although plasmids can be successfully conjugated into the...
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Induction of microsomal membrane proteins in roots of an aluminum-resistant cultivar of Triticum aestivum L. under conditions of aluminum stress
Download1994
Basu, U., Basu, A., Taylor, G. J.
Three-day-old seedlings of an Al-sensitive (Neepawa) and an Al-resistant (PT741) cultivar of Triticum aestivum were subjected to Al concentrations ranging from a to 100 mu M for 72 h. At 25 mu M Al, growth of roots was inhibited by 57% in the Al-sensitive cultivar, whereas root growth in the...
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Large-scale proteome profile of the zebrafish (Danio rerio) gill for physiological and biomarker discovery studies
Download2009
De Souza, A. G., Goss, G. G., MacCormack, T. J., Wang, N., Li, L.
Abstract: Zebrafish are an important model in vertebrate genetics, developmental biology, physiology, and toxicology. In this study, we established the first large-scale proteome profile of a teleost fish tissue using a shotgun method based on two-dimensional liquid chromatography-electrospray...
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Modulation of citrate metabolism alters aluminum tolerance in yeast and transgenic canola overexpressing a mitochondrial citrate synthase
Download2003
Basu, U., Anoop, V. M., Taylor, G. J., McAlister-Henn, L., McCammon, M. T.
Aluminum (Al) toxicity is a major constraint for crop production in acid soils, although crop cultivars vary in their tolerance to Al. We have investigated the potential role of citrate in mediating Al tolerance in Al-sensitive yeast (Saccharomyces cerevisiae; MMYO11) and canola (Brassica napus...