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- 3Liquid sample DESI-MS
- 2Electrospray Ionization
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- 2Protein-Ligand Interactions
- 2Protein–ligand affinities
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Application of Electrospray Ionization Mass Spectrometry to Study Protein-Ligand Interactions and Enzyme Kinetics
DownloadFall 2015
This thesis describes the development and application of electrospray ionization mass spectrometry (ESI-MS) to study the noncovalent protein-ligand interactions and also investigate the kinetic of enzymatic reactions. The rate of glycolipids cleavage by the human neuraminidase 3 (hNEU3) was...
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Fall 2016
This thesis describes the development of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was first applied to quantify protein-carbohydrate interactions in aqueous ammonium acetate...
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2016
Klassen, John S., Yao, Yuyu, Kitova, Elena N., Richards, Michele R.
The results of an investigation into the influence of sulfolane, a commonly used supercharging agent, on electrospray ionization mass spectrometry (ESI-MS) measurements of protein–ligand affinities are described. Binding measurements carried out on four protein–carbohydrate complexes, lysozyme...
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2016
Klassen, John S., Yao, Yuyu, Kitova, Elena N., Richards, Michele R.
The results of an investigation into the influence of sulfolane, a commonly used supercharging agent, on electrospray ionization mass spectrometry (ESI-MS) measurements of protein–ligand affinities are described. Binding measurements carried out on four protein–carbohydrate complexes, lysozyme...