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Skip to Search Results- 8Mass-spectrometry
- 7Electrospray ionization
- 5Isotope labeling
- 3Glycolipid
- 2Affinity
- 2Charging mechanism
- 4Kitova, Elena N.
- 4Klassen, John S.
- 3Han, Ling
- 2Johnson, M. A., Eugenio, Luiz
- 2Lin, Hong
- 2Ng, Kenneth K.
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2016
Klassen, John S., Han, Ling, Kitova, Elena N.
This study reports on the use of the catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) assay, combined with glycomicelles, as a method for detecting specific interactions between water-soluble proteins and glycolipids (GLs) in aqueous solution. The B subunit homopentamers...
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2016
Han, Ling, Klassen, John S., Kitova, Elena N.
This study reports on the use of the catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) assay, combined with glycomicelles, as a method for detecting specific interactions between water-soluble proteins and glycolipids (GLs) in aqueous solution. The B subunit homopentamers...
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Development and Application of ESI-MS Based Techniques for Quantitative Determination of Protein-Carbohydrate Interactions
DownloadFall 2016
This thesis describes the development and application of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was employed to quantify protein-carbohydrate interactions in aqueous buffer...
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Development and Applications of Chemical Isotope Labeling Methods for Metabolome Sample Normalization and Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadFall 2015
Metabolomics refers to the global study of the metabolome of a biological system where comprehensive and systematic profiling of all metabolites in a given biological sample (i.e., the metabolome) is performed. As the end product of biological processes, the status of metabolites could reflect...
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Development and Applications of Stable Isotope Labelling Liquid Chromatography Mass Spectrometry for Quantitative Proteomics
DownloadSpring 2011
This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...
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Development of Isotope Labeling Liquid Chromatography Mass Spectrometry for Biofluid Metabolomics and Applications in Disease Studies
DownloadSpring 2014
Metabolomics is a research field focusing on global study of all the metabolites present in a biological system (i.e., the metabolome). Metabolome analysis involves identifying and quantifying as many small molecule metabolites as possible in a biological sample. Metabolomics has attracted much...
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Development of Isotope Labeling Methods and Data Processing Program for Liquid Chromatography Mass Spectrometry-Based Metabolome Analysis
DownloadSpring 2014
Metabolomics strives to gain a comprehensive picture of the metabolites in a biological system. This emerging research field gives us an insight into the discovery of disease-related biomarkers and an understanding of metabolic flux as well as mechanisms. Liquid chromatography mass spectrometry...
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Development of New Late-Stage Labeling Methods with Labeled Carbon for Stabilized Carboxylic Acids and α-Amino Acids
DownloadFall 2023
Isotope labeling of small molecules is a crucial tool for drug discovery and understanding biochemical processes. Expanding the toolbox of a radiochemist with methods that allow late-stage labeling is therefore important. Of the methods that have been developed, the use of isotopically labeled...
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2012
Klassen, John S., Lin, Hong, Kitova, Elena N., Ng, Kenneth K., Johnson, M. A., Eugenio, Luiz
Electrospray ionization mass spectrometry (ESI-MS) measurements were performed under a variety of solution conditions on a highly acidic sub-fragment (B3C) of the C-terminal carbohydrate-binding repeat region of Clostridium difficile toxin B, and two mutants (B4A and B4B) containing fewer acidic...
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2012
Klassen, John S., Lin, Hong, Kitova, Elena N., Ng, Kenneth K., Johnson, M. A., Eugenio, Luiz
Electrospray ionization mass spectrometry (ESI-MS) measurements were performed under a variety of solution conditions on a highly acidic sub-fragment (B3C) of the C-terminal carbohydrate-binding repeat region of Clostridium difficile toxin B, and two mutants (B4A and B4B) containing fewer acidic...