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Skip to Search Results- 22Carbohydrates
- 22Electrospray ionization mass spectrometry
- 8Affinity
- 6Norovirus
- 5Library screening
- 4Lipids
- 26Chemistry, Department of
- 26Chemistry, Department of/Journal Articles (Chemistry)
- 11Graduate and Postdoctoral Studies (GPS), Faculty of
- 11Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 1Agricultural, Food and Nutritional Science, Department of
- 1Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
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Spring 2010
Interest in microfluidic platforms has surged as an alternative for sample preparation in the past two decades, with the potential for miniaturization, portability, automation, integration and parallelism driving this research. However, it is still very challenging to develop an integrated...
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Dissociation kinetics of the streptavidin-biotin interaction measured using direct electrospray ionization mass spectrometry analysis
Download2013
Kitova, Elena N., Klassen, John S., Deng, Lu
Dissociation rate constants (koff) for the model high affinity interaction between biotin (B) and the homotetramer of natural core streptavidin (S4) were measured at pH 7 and temperatures ranging from 15 to 45 °C using electrospray ionization mass spectrometry (ESI-MS). Two different approaches...
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Dissociation kinetics of the streptavidin-biotin interaction measured using direct electrospray ionization mass spectrometry analysis
Download2013
Deng, Lu, Klassen, John S., Kitova, Elena N.
Dissociation rate constants (koff) for the model high affinity interaction between biotin (B) and the homotetramer of natural core streptavidin (S4) were measured at pH 7 and temperatures ranging from 15 to 45 °C using electrospray ionization mass spectrometry (ESI-MS). Two different approaches...
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Energy use by the mountain pine beetle (Coleoptera: Curculionidae: Scolytinae) for dispersal by flight
Download2019-01-01
Wijerathna, Asha, Evenden, Maya
The mountain pine beetle, Dendroctonus ponderosae Hopkins is a major native pest of pine (Pinus Linnaeus (Pinaceae)) in western North America. Host colonization by mountain pine beetle is associated with an obligatory dispersal phase, during which beetles fly in search of a suitable host....
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Exploration of specific carbohydrate epitopes in their native habitat with the Staudinger ligation
DownloadFall 2011
Glycans modulate biological processes and play a vital role in the function of receptors. However, the structure and functions of glycans are often obscured due to the heterogeneity of these structures. We hypothesize that the function of a specific carbohydrate epitope can be elucidated by...
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Spring 2015
Acinetobacter baumannii remains a poorly understood, but very dangerous opportunistic pathogen. Though it lacks clear virulence traits expected of pathogens, it none the less poses a massive threat to the hospital system due to its inherent and acquired resistance to antibiotics, survival on...
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Identifying carbohydrate ligands of a norovirus P particle using a catch and release electrospray ionization mass spectrometry assay
Download2014
Jiang, Xi, Kitova, Elena N., Klassen, John S., Tan, Ming, Han, Ling
Noroviruses (NoVs), the major cause of epidemic acute gastroenteritis, recognize human histo-blood group antigens (HBGAs), which are present as free oligosaccharides in bodily fluid or glycolipids and glycoproteins on the surfaces of cells. The subviral P particle formed by the protruding (P)...
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Identifying carbohydrate ligands of a norovirus P particle using a catch and release electrospray ionization mass spectrometry assay
Download2014
Jiang, Xi, Kitova, Elena N., Klassen, John S., Tan, Ming, Han, Ling
Noroviruses (NoVs), the major cause of epidemic acute gastroenteritis, recognize human histo-blood group antigens (HBGAs), which are present as free oligosaccharides in bodily fluid or glycolipids and glycoproteins on the surfaces of cells. The subviral P particle formed by the protruding (P)...
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Measuring positive cooperativity using the direct ESI-MS assay: Cholera toxin B subunit homopentamer binding to GM1 pentasaccharide
Download2014
Lin, Hong, Klassen, John S., Kitova, Elena N.
Direct electrospray ionization mass spectrometry (ESI-MS) assay was used to investigate the stepwise binding of the GM1 pentasaccharide β-D-Galp-(1→3)-β-D-GalpNAc-(1→4)[α-D-Neu5Ac-(2→3)]-β-D-Galp-(1→4)-β-D-Glcp (GM1os) to the cholera toxin B subunit homopentamer (CTB5) and to establish...
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Measuring positive cooperativity using the direct ESI-MS assay: Cholera toxin B subunit homopentamer binding to GM1 pentasaccharide
Download2014
Kitova, Elena N., Lin, Hong, Klassen, John S.
Direct electrospray ionization mass spectrometry (ESI-MS) assay was used to investigate the stepwise binding of the GM1 pentasaccharide β-D-Galp-(1→3)-β-D-GalpNAc-(1→4)[α-D-Neu5Ac-(2→3)]-β-D-Galp-(1→4)-β-D-Glcp (GM1os) to the cholera toxin B subunit homopentamer (CTB5) and to establish...