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1999
Owttrim, George W., Magee, Wendy C., Yu, Esther, Chamot, Danuta
The ability to modify RNA secondary structure is crucial for numerous cellular processes. We have characterized two RNA helicase genes, crhB and crhC, which are differentially expressed in the cyanobacterium Anabaena sp. strain PCC 7120. crhC transcription is limited specifically to cold shock...
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Crystallization and preliminary diffraction studies of TraF, a component of the Escherichia coli type IV secretory system
Download2004
Manchak, J., Hayakawa, K., Audette, G.F., Holland, S.J., Frost, L.S., Hazes, B.
TraF, a component of the Escherichia coli type IV secretory system, has been crystallized and preliminary X-ray diffraction data have been collected. TraF is a 26 kDa protein encoded by the E. coli F plasmid and is required for conjugative plasmid transfer and the formation of sex pili. The...
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Differential exudation of polypeptides by roots of aluminum-resistant and aluminum-sensitive cultivars of Triticum aestivum L. in response to aluminum stress
Download1994
Basu, A., Taylor, G. J., Basu, U.
Cultivars of Triticum aestivum differing in resistance to Al were grown under aseptic conditions in the presence and absence of Al and polypeptides present in root exudates were collected, concentrated, and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Upon exposure to...
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Drosophila ATM and ATR have distinct activities in the regulation of meiotic DNA damage and repair
Download2011
Tiong, S., McKim, K.S., Campbell, S.D., White-Brown, S.K., Paul, A., Pedersen, M., Joyce, E.F.
Ataxia telangiectasia–mutated (ATM) and ataxia telangiectasia–related (ATR) kinases are conserved regulators of cellular responses to double strand breaks (DSBs). During meiosis, however, the functions of these kinases in DSB repair and the deoxyribonucleic acid (DNA) damage checkpoint are...
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1999
Tenniswood, M., Bennett, S., Wong, P., Morrissey, C., Nitsche, E., Guenette, R.S.
After hormonal ablation, 90% of the secretory epithelial cells of the prostate undergo apoptosis, and the remaining cells are reorganized as the tissue is remodeled. Using differential display RT-PCR of total RNA extracted from the rat ventral prostate before and 4 days after castration, we have...
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Gawky Is a Component of Cytoplasmic mRNA Processing Bodies Required for Early Drosophila Development
Download2006
Hobman, T. C., Locker, J., Schneider, M. D., Pare, J. M., Haskins, J., Simmonds, A. J., Chaker, S., Najand, N
In mammalian cells, the GW182 protein localizes to cytoplasmic bodies implicated in the regulation of messenger RNA ( mRNA) stability, translation, and the RNA interference pathway. Many of these functions have also been assigned to analogous yeast cytoplasmic mRNA processing bodies. We have...
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2001
Bohnert, H. J., Deyholos, M., Brazille, S., Wang, H., Kawai, K., Borchert, C., Galbraith, D., Kawasaki, S.
Transcript regulation in response to high salinity was investigated for salt-tolerant rice (var Pokkali) with microarrays including 1728 cDNAs from libraries of salt-stressed roots. NaCl at 150 mM reduced photosynthesis to one tenth of the prestress value within minutes. Hybridizations of RNA to...
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Genomic sequence and activity of KS10, a transposable phage of the Burkholderia cepacia complex
Download2008
Seed, K.D., Lynch, K.H., Dennis, J.J., Goudie, A.D., Wishart, D.S., Shrivastava, S., Stothard, P.
Background: The Burkholderia cepacia complex (BCC) is a versatile group of Gram negative organisms that can be found throughout the environment in sources such as soil, water, and plants. While BCC bacteria can be involved in beneficial interactions with plants, they are also considered...
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Induction of microsomal membrane proteins in roots of an aluminum-resistant cultivar of Triticum aestivum L. under conditions of aluminum stress
Download1994
Basu, U., Basu, A., Taylor, G. J.
Three-day-old seedlings of an Al-sensitive (Neepawa) and an Al-resistant (PT741) cultivar of Triticum aestivum were subjected to Al concentrations ranging from a to 100 mu M for 72 h. At 25 mu M Al, growth of roots was inhibited by 57% in the Al-sensitive cultivar, whereas root growth in the...
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Modulation of citrate metabolism alters aluminum tolerance in yeast and transgenic canola overexpressing a mitochondrial citrate synthase
Download2003
Basu, U., Anoop, V. M., Taylor, G. J., McAlister-Henn, L., McCammon, M. T.
Aluminum (Al) toxicity is a major constraint for crop production in acid soils, although crop cultivars vary in their tolerance to Al. We have investigated the potential role of citrate in mediating Al tolerance in Al-sensitive yeast (Saccharomyces cerevisiae; MMYO11) and canola (Brassica napus...