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Skip to Search Results- 19Biosensors
- 15Fluorescence
- 12Fluorescent proteins
- 4Protein engineering
- 2Cantilever
- 2Directed evolution
- 2El-Yazbi, Amira F.
- 2Loppnow, Glen R.
- 2Zhang, Wei
- 1Abdelfattah, Ahmed
- 1Alford, Spencer Caleb
- 1Anbukarasu, Preetam
- 34Graduate and Postdoctoral Studies (GPS), Faculty of
- 34Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 3Chemistry, Department of
- 3Chemistry, Department of/Journal Articles (Chemistry)
- 1Agricultural, Food and Nutritional Science, Department of
- 1Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
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Spring 2015
Fluorescent proteins (FP) and FP-based biosensors have become essential tools for cell biology and neuroscience research. This thesis describes efforts to engineer new FPs with red emission and novel genetically encoded biosensors based on red FPs. Directed evolution and semi-rational design are...
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Fall 2015
The expansion of the fluorescent protein (FP) colour palette through discovery of more species possessing FPs and by subjecting them to intensive protein engineering efforts has greatly aided the effectiveness of FP-based biosensor designs. Currently, the main FP-based methods for detecting...
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Expanding the optogenetic toolkit with a photocleavable protein and a near infrared pH indicator
DownloadFall 2017
Optogenetics is an emerging biological technique that is causing a revolution in life science. It uses optical methods to control and visualize genetically targeted biological events in a relatively non-invasive and spatiotemporally precise manner. Optogenetics requires advanced optical...
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Spring 2016
This thesis explores the dynamics and sensing performance of silicon quantum dot (SiQD) ensembles coupled to the whispering gallery modes (WGMs) of spherical and cylindrical microcavities. The first project investigated the possibility of observing Purcell effects in an ensemble of QDs coupled to...
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Integration of Nanomechanical Resonators in Microfluidic Systems for Specific Protein Detection
DownloadFall 2009
Nanomechanical resonators offer a pathway towards highly sensitive and label-free detection of biomolecules by transducing the mass of bound analytes into resonant frequency shifts. Zeptogram level and even single cell detections have been successfully achieved by others. However, these...
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Investigation on the uptake of functional proteins and infectious prions into wheat plants through the root system
DownloadSpring 2014
Prions are the proteinaceous particle responsible for infections in a class of neurodegenerative diseases. These diseases affect a number of mammals including cervids where it is termed Chronic Wasting Disease (CWD). Prions enter the environment and persist for years. Plants have the ability to...
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Spring 2013
Absorption of UV light by nucleic acids can result in the formation of molecular lesions leading to mutagenesis, carcinogenesis, and cell death. Thus, understanding DNA damage is important for elucidating the molecular mechanisms of disease. Much effort has been focused on developing methods for...
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Fall 2013
Metal-dielectric thin film multilayers are of significant interest as transparent conductors, band-pass filters, and metamaterials. This thesis describes an experimental study of optical tunnelling through periodic metal-dielectric (MD) multilayers with the goal of optimizing the transmittance...
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Fall 2016
The field of conducting polymers and mainly organic electrochemical transistors (OECT) have been investigated for bioelectronics applications. Advantages such as conformability, high quality electrical contact, ease of fabrication and biocompatibility made OECTs a great potential of future bio...
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Spring 2011
Intracellular pH was monitored at the cytosolic surface of plasma membrane solute transporters (Na+/H+/nucleoside co-transporters, or Cl-/HCO3- exchangers), using pH-sensitive fluorescent proteins (FPs), dual emission green FP (deGFP4) and a monomeric red FP Nectarine (mNect), whose development...