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- 3Chemistry, Department of/Journal Articles (Chemistry)
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Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method
Download2009
Kitova, Elena N., Sun, Jiangxiao, Sun, Nian, Klassen, John S.
The application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes...
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Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method
Download2009
Sun, Nian, Klassen, John S., Kitova, Elena N., Sun, Jiangxiao
The application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes...
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Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method
2009
Kitova, Elena N., Klassen, John S., Sun, Nian, Sun, Jiangxiao
The application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes...
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Improving the accuracy of protein secondary structure prediction using structural alignment
Download2006
Gallin, W.J., Wishart, D.S., Montgomerie, S., Sundararaj, S.
Background: The accuracy of protein secondary structure prediction has steadily improved over the past 30 years. Now many secondary structure prediction methods routinely achieve an accuracy (Q3) of about 75%. We believe this accuracy could be further improved by including structure (as opposed...
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2007
Sperling, F. A. H., MacQuarrie, C. J. K., Langor, D. W.
Mitochondrial cytochrome oxidase I and 11 genes were sequenced for two invasive alien birch (Betula L. [Betulaceae]) leaf-mining sawflies, Profenusa thomsoni (Konow, 1886) (Hymenoptera: Tenthredinidae) and Scolioneura betuleti (King, 1816) (Hymenoptera: Tenthredinidae), accidentally introduced...
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Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera
Download2020-02-01
Wong, Barbara, Leal, Isabel, Feau, Nicolas, Dale, Angela, Uzunovic, Adnan, Hamelin, Richard C.
Wood and wood products can harbor microorganisms that can raise phytosanitary concerns in countries importing or exporting these products. To evaluate the efficacy of wood treatment on the survival of microorganisms of phytosanitary concern the method of choice is to grow microbes in petri dishes...
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Molecular cloning of cDNA encoding a 55-kDa multifunctional thyroid hormone binding protein of skeletal muscle sarcoplasmic reticulum
Download1990-01-01
Fliegel, Larry, Newton, E., Burns, K., Michalak, M.
A cDNA clone encoding 55-kDa multifunctional, thyroid hormone binding protein of rabbit skeletal muscle sarcoplasmic reticulum was isolated and sequenced. The cDNA encoded a protein of 509 amino acids, and a comparison of the deduced amino acid sequence with the NH2-terminal amino acid sequence...