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Skip to Search Results- 3Hwang, Peter M.
- 3Liu, Philip B.
- 2Zahran, Somaya
- 1Chan, Brandon Y.H.
- 1Danmaliki, Gaddafi I.
- 1Davies, Peter L.
- 1Bacterial amino acid biosynthetic pathways
- 1Calpain
- 1Central metabolic pathways
- 1Chemical cleavage
- 1Escherichia coli
- 1Fusion protein expression
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Combining a PagP fusion protein system with nickel ion-catalyzed cleavage to produce intrinsically disordered proteins in E. coli
Download2015-01-01
Zahran, Somaya, Pan, Jonathan S., Liu, Philip B., Hwang, Peter M.
Many proteins contain intrinsically disordered regions that are highly solvent-exposed and susceptible to post-translational modifications. Studying these protein segments is critical to understanding their physiologic regulation, but proteolytic degradation can make them difficult to express and...
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Stereo-selective deuteration in aspartate, asparagine, lysine, and methionine amino acid residues using fumarate as a carbon source for E. coli in D2O
Download2017-01-01
Danmaliki, Gaddafi I., Liu, Philip B., Hwang, Peter M.
We propose an isotope labeling strategy for expressing proteins in E. coli using protonated natural abundance 12C carbon sources in D2O. The strategy eliminates dominant 1H-13C and 1H-1H dipolar relaxation mechanisms to produce long-lived magnetization for solution NMR spectroscopy. Isolated 1H...
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Structure and proteolytic susceptibility of the inhibitory C-terminal tail of cardiac troponin I
Download2019-01-01
Mahmud, Zabed, Zahran, Somaya, Liu, Philip B., Reiz, Bela, Chan, Brandon Y.H., Roczkowsky, Andrej, McCartney, Christian-Scott E., Davies, Peter L., Li, Liang, Schulz, Richard, Hwang, Peter M.
Background Cardiac troponin I (cTnI) has two flexible tails that control the cardiac cycle. The C-terminal tail, cTnI135-209, binds actin to shut off cardiac muscle contraction, whereas the competing calcium-dependent binding of the switch region, cTnI146-158, by cardiac troponin C (cTnC)...