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- 1Membrane Proteins
- 1Myocardial ischemia-reperfusion injury
- 1Nuclear MMP-2
- 1Nuclear Proteins
- 1Nuclear matrix metalloproteinase-2
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Nuclear matrix metalloproteinase-2 and investigation of its potential targets in myocardial ischemia-reperfusion injury
DownloadSpring 2016
Matrix metalloproteinases (MMPs) are zinc-dependent proteases involved in intra- and extra-cellular matrix remodeling. MMP-2 was the first to be localized to the nucleus; however the biological functions and substrates of nuclear MMP-2 are mostly unknown. We hypothesized that...
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Sequential fractionation and isolation of subcellular proteins from tissue or cultured cells
Download2015-01-01
Baghirova, Sabina, Hughes, Bryan G., Hendzel, Michael J., Schulz, Richard
Many types of studies require the localization of a protein to, or isolation of enriched protein from a specific cellular compartment. Many protocols in the literature and from commercially available kits claim to yield pure cellular fractions. However, in our hands, the former often do not work...