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Developing General Methods for Studying Enzymatic Reactions at Symmetric Bilayers and Nucleic Acid Triggered Lesion Induced DNA Amplification

  • Author / Creator
    Li, Yimeng
  • Understanding molecular recognition is essential to biological and material applications. Surface-specific techniques based on second order nonlinear optical processes such as second harmonic generation (SHG) and sum frequency generation (SFG) are able to selectively study biological events occurring at the interface. We used SFG to probe the Phospholipase D induced hydrolysis reaction of symmetric lipid bilayer supported on a planar silica surface. The χ(3) SHG technique was used as a complementary technique to monitor the reaction due to changes in interfacial potential of the bilayer after hydrolysis. Based on our group’s previous work, isothermal turnover can be achieved in DNA-templated ligation reactions using destabilizing modifications to the DNA. This system was applied to the detection of target sequences specific to the Hepatitis B virus. The generality of lesion-induced isothermal DNA amplification was also expanded to RNA detection.

  • Subjects / Keywords
  • Graduation date
    2013-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3G737991
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Chemistry
  • Supervisor / co-supervisor and their department(s)
    • Gibbs-Davis, Julianne M. (Chemistry)
  • Examining committee members and their departments
    • Klassen, John (Chemistry)
    • Petersen, Nils O. (Chemistry)