Alteration of liver fat metabolism following irinotecan plus 5-fluorouracil treatment

  • Author / Creator
    Pant, Asha
  • This study determined how chemotherapy treatment for colorectal cancer alters hepatic fat metabolism. Livers were isolated from tumor-bearing animals after one and two cycles of chemotherapy consisting of irinotecan plus 5-fluorouracil. Fatty acid amounts and composition within triglycerides (TG) and phospholipids (PL) as well as gene expression were assessed. Total TG did not change and total PL were higher after 7 days following treatment. Docosahexaenoic acid became undetectable after the treatment at 5 and 7 days, respectively. Following one cycle, the alterations appeared temporary. After second cycle, total TG were lower and n-3 fatty acids were also lower causing n-6/n-3 ratio to be higher. Chemotherapy altered 13 genes of 44, however not all genes within a single pathway were affected. The alterations in gene expression did not necessarily parallel the observations in fatty acids. This study is important to develop identify target pathways to circumvent negative effects of chemotherapy-associated steatohepatitis.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
    • Department of Agricultural, Food and Nutritional Science
  • Supervisor / co-supervisor and their department(s)
    • Dr Vera Mazurak, Agricultural, Food and Nutritional Science
  • Examining committee members and their departments
    • Dr Jason Yap, Department of Medicine
    • Dr Vickie Baracos, Oncology and Agricultural, food and Nutrition Science
    • Dr Rene Jacobs, Agricultural, food and Nutritional Science