High-throughput, high-sensitivity analysis of gene expression in Arabidopsis

• Author(s) / Creator(s)

  • Seligmann, B.
  • Deyholos, M.
  • Kris, R. M.
  • Felder, S.
  • Botros, I.
  • Galbraith, D. W.
  • Martel, R.
  • Hinton, J.
  • Lambert, G. M.

• High-throughput gene expression analysis of genes expressed during salt stress was performed using a novel multiplexed quantitative nuclease protection assay that involves customized DNA microarrays printed within the individual wells of 96-well plates. The levels of expression of the transcripts from 16 different genes were quantified within crude homogenates prepared from Arabidopsis (Arabidopsis thaliana) plants also grown in a 96-well plate format. Examples are provided of the high degree of reproducibility of quantitative dose-response data and of the sensitivity of detection of changes in gene expression within limiting amounts of tissue. The lack of requirement for RNA purification renders the assay particularly suited for high-throughput gene expression analysis and for the discovery of novel chemical compounds that specifically modulate the expression of endogenous target genes.

• Date created

  2007

• Subjects / Keywords

  • Pathways
  • Thaliana
  • Responsive Genes
  • Genome
  • Array
  • High-Salinity
  • Length CDNA Microarray
  • Discovery
  • Oxidative Stress
  • Profiles

• Type of Item

  Article (Published)

• DOI

  https://doi.org/10.7939/R38K75327

• License

  © 2007 American Society of Plant Biologists. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.