Investigation of osteogenic and adhesive properties of Rosette nanotubes

  • Author / Creator
    Agrawal, Rahul
  • This thesis is focussed on bone tissue engineering for improving the osseointegration of joint implants. We have incorporated nano-scaled materials called as Rosette Nanotubes (RNTs) which mimic the geometry of the natural components of bone with the growth factor, Bone Morphogenetic Protein-7 (BMP-7). In particular, short peptide sequences were identified from BMP-7 (SNVILKKYRN, KPSSAPTQLN, and KAISVLYFDDS) and were linked with Twin K1 RNTs. The first stage of our study was focused on the purification and characterization of these short peptides co-assembled with RNTs using HPLC (High Performance Liquid Chromatography) and their characterization by SEM (Scanning Electron Microscopy) respectively. The Second stage involved cyto-compatibility studies of the RNTs. The third stage involved the adhesion studies of RNTs coated on different substrates. The fourth and final stage was the osteogenic differentiation studies of these materials. Results have shown that the HPLC purification yield was 12-20% and SEM characterization showed the presence of successfully assembled nanotubes. Coating of RNTs on different substrates has shown a significant increase in adhesion of human Bone Marrow Stromal Cells (hBMSCs) on tissue culture treated plates and non-tissue culture treated plates. Moreover, peptide co-assemblies with Twin K1 RNTs have shown a significant increase in specific ALP (Alkaline phosphatase) activity. In summary, Twin K1 RNTs and peptide co-assembled RNTs have shown promising results for improving the cell adhesion on plastic substrates (treated and non-treated plates). Moreover, peptide co-assembled RNTs have shown early signs of osteogenic differentiation.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
    • Department of Biomedical Engineering
  • Specialization
    • Biomedical Sciences
  • Supervisor / co-supervisor and their department(s)
    • Fenniri, Hicham (Chemistry), Supervisor
  • Examining committee members and their departments
    • Jomha, Nadr (Surgery), Committee member
    • Uludag, Hasan (Chemical and Materials engineering), co-Supervisor
    • Burrell, Robert (Biomedical Engineering), Committee Chair