Electrografted Thick Diazonium Derived Films for Biosensing Applications

  • Author / Creator
    Toman, John T
  • Electrografting permits bonding of an organic film to a conductive substrate. It is therefore important to control the formation of organic films as best as possible and to understand the linkage between the organic film and the substrate as best as possible. This work explores electrografting of diazonium salts using high reduction potentials to prepare thick aryl films as substrates for SPR immunoassays. Film thickness was linear with respect to applied reduction potential for the modification of gold electrodes with phenylacetic acid and nitroazobenzene diazonium salts. Further, the presence of redox active functional groups was determined to be unnecessary due to the large driving force of the reaction. Phenylacetic acid films were shown to provide a suitable platform for antibody immobilization and antigen binding, with LOD’s comparable to other SPR based biosensors. The immobilization of antibodies and subsequent antigen binding was shown to be highly dependent on surface morphology.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
  • Supervisor / co-supervisor and their department(s)
  • Examining committee members and their departments
    • Campbell, Robert (Chemistry)
    • Serpe, Michael (Chemistry)