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Development and Analysis of Magnetic Carbon Nanotube and Microbubble-Assisted High Centrifugation Field Techniques For The Increase of Cell Membrane Permeability

  • Author / Creator
    He, Chuan
  • To cure diseases at the gene level, therapeutic biomaterials need to enter the cells. However, since the cell membrane is a highly selective structure, its permeability is usually very low. In this thesis, we propose two innovative and efficient methods to increase cell membrane permeability. The first method is based on magnetic carbon nanotubes (mCNT). By using atomic force microscope force curve analysis (AFM-FCA) and transmission electron microscope (TEM) images, we successfully develop mCNT from raw materials. Under a magnetic field, mCNT can facilitate cell endocytosis by firmly attaching onto the cell membrane. As a result, the cell membrane permeability of several mammalian cell lines is increased. The second method to increase cell membrane permeability is based on using microbubble-assisted high centrifugation field. The mechanism of this method relies on the centrifugation-induced collision between microbubbles and cells and the force of the microbubbles bursting. The validity of this method is demonstrated on mammalian cells and plant cells. Theoretical models are built to simulate the interaction between microbubbles and cells in the centrifugation field. The simulation results indicate that intracellular pathways can be created once the relative velocity between the microbubble and cell is beyond a critical value. In addition, cell deformed morphology induced in the centrifugation field and cell mechanical properties are closely related to the resulting increase of cell membrane permeability.

  • Subjects / Keywords
  • Graduation date
    2014-06
  • Type of Item
    Thesis
  • Degree
    Doctor of Philosophy
  • DOI
    https://doi.org/10.7939/R3VH5CS2H
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Doctoral
  • Department
    • Department of Electrical and Computer Engineering
  • Specialization
    • Biomedical Engineering
  • Supervisor / co-supervisor and their department(s)
    • Chen, Jie
  • Examining committee members and their departments
    • Datla, Raju (National Research Council)
    • Zhao, Vicky (Electrical and Computer Engineering)
    • Zeng, Hongbo (Chemical and Materials Engineering)
    • Chen, Jie (Electrical and Computer Engineering)
    • Dew, Steve (Electrical and Computer Engineering)