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Improvement of functionality of barley protein by deamidation

  • Author / Creator
    Zhao, Jing
  • In this study, the deamidation is involved to modify the structure of barley proteins in terms of prolamin and glutelin in order to improve the functional properties of protein. A wide range of deamidation degrees (0.1% to 45%) were prepared using alkaline method. The results suggested that the optimal deamidation degree of barley prolamin is around 2.4-4.7%, where the solubility, emulsifying and foaming properties of prolamin were significantly improved at both acidic and neutral pHs. The optimal deamidation degree for glutelin is around 2.2 to 5.6%, where deamidated glutelin demonstrated markedly improved solubility at both acidic and neutral pHs. Glutelin performed strong tendency to form aggregates with spherical shape and very large molecular weight. These aggregates are important in stabilizing the emulsions at a broad range of deamidation degree (5.6-43%). These results suggest that barley protein would be an excellent candidate to develop as an emulsifying and foaming ingredient.

  • Subjects / Keywords
  • Graduation date
    2011-06
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3W43F
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Agricultural, Food and Nutritional Science
  • Supervisor / co-supervisor and their department(s)
    • Lingyun Chen, Department of Agricultural, Food and Nutritional Science)
  • Examining committee members and their departments
    • Michael Gaenzle, Department of Agricultural, Food and Nutritional Science
    • Zhenghe Xu, Department of Chemical and Materials Engineering