Communities and Collections
Usage
  • 64 views
  • 52 downloads

Type 2 porcine reproductive and respiratory syndrome virus infection increases apoptosis at the maternal-fetal interface in late gestation pregnant gilts

  • Author(s) / Creator(s)
  • The pathogenesis of fetal death associated with porcine reproductive and respiratory syndrome
    (PRRS) is hypothesized to be a consequence of PRRS virus-induced apoptosis at
    the maternal-fetal interface (MFI). The objectives of this study were to evaluate distribution
    and degree of apoptosis in the uterine and fetal placental tissues during the experimental
    type 2 PRRS virus (PRRSV) infection and determine associations between apoptosis at the
    MFI, PRRSV RNA concentration and antigen staining intensity, PRRSV-induced microscopic
    lesions, and fetal preservation status. A total of 114 naïve, high-health pregnant gilts
    were inoculated with type 2 PRRSV on gestation day 85±1 with euthanasia 21 days later; 19
    sham-inoculated gilts served as controls. Two hundred and fifty samples of uterine tissue
    with fetal placenta were selected based on negative, low PRRSV RNA, and high PRRSV
    RNA concentration (0, < or > 2.7 log10 copies/mg, respectively). TUNEL assay was used to
    detect apoptosis in the endometrium and at the MFI. PRRSV RNA concentration and numbers
    of PRRSV immunopositive cells in uterine and placental tissue were positively associated
    with the severity of apoptosis in the endometrium and the MFI (P<0.001, P<0.05 and
    P<0.001, respectively). The number of TUNEL positive cells at the MFI was also positively
    associated with the severity (P<0.001) of vasculitis, but not total numbers of inflammatory
    cells in the endometrium. Increased numbers of TUNEL positive cells at the MFI were associated
    with PRRSV load in the fetal thymus, and greater odds of meconium staining of the
    fetus at 21 days post infection (P<0.001 for both). These findings suggest an important role
    of apoptosis in the pathogenesis of uterine epithelial and trophoblastic cell death at the MFI.
    Moreover, apoptosis at the MFI is significantly associated with fetal demise during in utero
    type 2 PRRSV infection.

  • Date created
    2017-03-01
  • Subjects / Keywords
  • Type of Item
    Article (Published)
  • DOI
    https://doi.org/10.7939/r3-q12w-dv87
  • License
    Attribution-NonCommercial 4.0 International
  • Language
  • Citation for previous publication
    • Novakovic P, Harding JCS, Al-Dissi AN, Detmer SE (2017) Type 2 porcine reproductive and respiratory syndrome virus infection increases apoptosis at the maternal-fetal interface in late gestation pregnant gilts. PLoS ONE 12(3): e0173360. doi:10.1371/journal.pone.0173360