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Fabrication of colloidal crystals featuring sub-20 nm pore size for protein separation

  • Author / Creator
    Malekpourkoupaei, Ali
  • Clinical point-of-care (POC) diagnosis remains an open and challenging field of research worldwide in which the study of the mechanism and dynamics of protein molecule separation using electrophoresis is of considerable interest. The quality of a protein separation is strongly dependent on the separating matrix and conditions under which the electrophoresis is performed. This research is mainly focused on development of a novel separation matrix, based upon the selfassembly of particles in a colloidal suspension, within a microfluidic chip. Several challenges were overcome in developing crystalline particle arrays that generate a porous material with pores smaller than 20 nm. The effect of pore size on separation of proteins of differing molecular weight and size was then studied, and the mechanism of separation, which appears to change in the smallest size range, was explored.

  • Subjects / Keywords
  • Graduation date
    2012-09
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3BV7B41N
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Mechanical Engineering
  • Supervisor / co-supervisor and their department(s)
    • Harrison, D. Jed
    • Kostiuk, Larry W.
  • Examining committee members and their departments
    • Olfert, Jason (Mechanical Engineering)
    • Kostiuk, Larry W. (Mechanical Engineering)
    • Harrison, D. Jed (Chemistry)