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Extraction of Triticale Distillers Grain Proteins for Adhesive Development
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- Author / Creator
- Bandara, Nandika Priyantha
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Triticale (×Triticosecale Wittmackk) is being actively explored as a feedstock for bioethanol production in Canada. As the main co-product of bioethanol processing, triticale distillers grain contains 20-43% protein (dry basis), and mainly used as animal feed. The purpose of this study was to find new uses of triticale protein as adhesive. Proteins from triticale distillers wet grains (DWG) and distillers dried grains with solubles (DDGS) were extracted by five methods: pH shifting method, 60% ethanol, alkaline ethanol, glacial acetic acid, and enzyme-aided extraction. Extraction with alkaline ethanol and glacial acetic acid gave higher protein contents (~61–65%) than those obtained from other extraction methods (~23–24%); however, enzyme-aided extraction using Protex 6L yielded 75–82% protein at a content of 43–57%. To prepare adhesives, proteins were modified by NaOH, urea and glutaraldehyde; effects of modifications on protein structure were analyzed by FTIR, and their adhesion properties were measured by automated bonding evaluation system (ABES II). The highest (p<0.05) adhesion strength was observed in acetic acid extracted proteins; glutaraldehyde modification acetic acid extracted proteins increased the adhesion strength from 2.56, 0.84, 1.11 MPa to 3.86, 2.03, 2.60 MPa for dry, wet and soaked adhesion strength, respectively. Increases in α-helical conformation and molecular weight were observed for glutaraldehyde modified proteins. Development of adhesive from triticale proteins might represent new uses of triticale distillers grain.
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- Subjects / Keywords
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- Graduation date
- Fall 2011
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- Type of Item
- Thesis
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- Degree
- Master of Science
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- License
- This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.