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Novel polymer and lipid-based nanocarriers for gene delivery

  • Author / Creator
    Fitzsimmons, Ross
  • The following thesis describes original studies assessing the gene delivery efficacy of novel non-viral carrier combinations. The first panel of non-viral carriers tested are termed AVPs (artificial viral particles), in reference to their structural similarity to enveloped viruses, and were fabricated in a composite manner using a variety of polymers and lipids in order to deliver plasmid DNA. The most effective carrier found in these studies was 25 kDa PEI (polyethylenimine), with none of the AVPs showing a clear superior effect to that of 25 kDa PEI. While a highly effective gene carrier, PEI is extremely cytotoxic and hence PEI was conjugated with PEG (polyethylene glycol) in attempt to decrease its toxicity while maintaining its functionality. Ultimately, it was found that PEGylation generally decreased the transfection efficacy of PEI, but under ideal conditions of PEG substitution and polymer/DNA (w/w) ratio, this decrease in efficacy can be circumvented.

  • Subjects / Keywords
  • Graduation date
    2012-06
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3CW7W
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Biomedical Engineering
  • Supervisor / co-supervisor and their department(s)
    • Uludag, Hasan (Chemical and Materials Engineering)
  • Examining committee members and their departments
    • Narain, Ravin (Chemical and Materials Engineering)
    • Unsworth, Larry (Chemical and Materials Engineering)