Non-invasive Label-free Cytometry Based on Laser Light Scattering Technique

  • Author / Creator
    Wan, Yu
  • A non-invasive label-free cytometry technique has been developed and advanced. This laser light scattering technique, alternative to the conventional fluorescent-activated flow cytometry (FACS) using biomarkers, identifies a micro scale particle or biological cell based on its two-dimensional scattered laser light patterns. Patterns have been obtained in three directions: forward, side and backward, and two different wavelengths (green and red) illumination. In this thesis, the label-free cytometry technique was first used to study different sizes of spherical and non-spherical beads, and then non-treated and staurosporine-treated SH-SY5Y cells. The experimental results from the spherical beads were compared to Mie theory. To analyze the patterns from the cells, pattern classification and recognition based on angle distribution method and finding local maximum method have been applied. Preliminary experiment with a capillary tube as well as particles scattering in a droplet which could help the design of practical cytometers have been carried out.

  • Subjects / Keywords
  • Graduation date
    Fall 2018
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.