New chromatin regulators contributing to the transcriptional control of HUG1

  • Author / Creator
    Walker, Amelia C
  • Chromatin structure is important aspect of transcriptional regulation. Replication-coupled (RC) nucleosome assembly is the process of depositing newly synthesized H3-H4 onto nascent DNA behind the replication fork, mediated by the histone chaperones Asf1, CAF-1, and Rtt106. The experiments described in thesis test our hypothesis that the RC chaperones contribute to the regulation of HUG1 due to the important role they play in RC nucleosome assembly and therefore chromatin structure. Collectively, research in this thesis provides evidence that CAF-1 and Rtt106 contribute to the repression of HUG1 in a way that is unrelated to its normal regulation. Interestingly, this repression does not involve Asf1, even though Asf1 functions upstream of these chaperones during RC nucleosome assembly. These results suggest divergent functions of the RC chaperones that differently affect the regulation of HUG1. These divergent functions of the RC chaperones also have opposing roles in promoting survival during prolonged replication stress.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
    • Department of Biochemistry
  • Supervisor / co-supervisor and their department(s)
    • Schultz, Michael (Biochemistry)
  • Examining committee members and their departments
    • Schang, Luis (Biochemistry)
    • Berry, Fred (Medical genetics)
    • Underhill, Alan (Medical genetics)
    • Schultz, Michael (Biochemistry)