Mutant Group B Streptococcus surface expressed Phosphoglycerate kinase (PGK) with reduced plasminogen binding

• Author / Creator

  Siddiqua, Khalida

• Group B streptococcus (GBS) is a Gram-positive streptococcus bacterium that can
  cause severe invasive disease in the human neonate. This can manifest as
  pneumonia, septicemia and meningitis. While antibiotic prophylaxis has reduced
  the incidence GBS disease in the neonatal population, it is still the one of the
  leading cause of neonatal invasive disease in North America including Alberta.
  Phosphoglycerate kinase (PGK), a glycolytic enzyme, has been demonstrated to
  be on the surface of GBS. Surface expressed GBS-PGK interacts with the hostcell
  protein plasminogen, which is thought to help bacteria invade further into the
  host by destruction of host barriers. In this thesis, a triple mutant GBS-PGK
  molecule PGK-M9 was created based on information from a space-filled model of
  PGK-plasminogen interaction sites. PGK-M9 bound 95% less plasminogen than
  the wild type GBS-PGK. This mutant will permit further investigation into the
  role of surface GBS-PGK in vivo models of GBS infection.

• Subjects / Keywords

  • Phosphoglycerate kinase

• Graduation date

  Spring 2014

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R38P5VJ33

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.