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Mutant Group B Streptococcus surface expressed Phosphoglycerate kinase (PGK) with reduced plasminogen binding
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- Author / Creator
- Siddiqua, Khalida
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Group B streptococcus (GBS) is a Gram-positive streptococcus bacterium that can
cause severe invasive disease in the human neonate. This can manifest as
pneumonia, septicemia and meningitis. While antibiotic prophylaxis has reduced
the incidence GBS disease in the neonatal population, it is still the one of the
leading cause of neonatal invasive disease in North America including Alberta.
Phosphoglycerate kinase (PGK), a glycolytic enzyme, has been demonstrated to
be on the surface of GBS. Surface expressed GBS-PGK interacts with the hostcell
protein plasminogen, which is thought to help bacteria invade further into the
host by destruction of host barriers. In this thesis, a triple mutant GBS-PGK
molecule PGK-M9 was created based on information from a space-filled model of
PGK-plasminogen interaction sites. PGK-M9 bound 95% less plasminogen than
the wild type GBS-PGK. This mutant will permit further investigation into the
role of surface GBS-PGK in vivo models of GBS infection. -
- Subjects / Keywords
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- Graduation date
- Spring 2014
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- Type of Item
- Thesis
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- Degree
- Master of Science
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- License
- This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.