Optimizing Cultural Conditions for Duct Cells

  • Author / Creator
    Ahmed, Mohammed
  • In-vitro expansion of the duct cells of the pancreas was associated with loss of the epithelial phenotype of these cells and acquisition of mesenchymal phenotype. These changes have been explained by epithelial mesenchymal transition. We have tested the effect of several growth factors that have been used in epithelial cell cultures with a number of different base media. We used geneticin that has been used to limit the growth of mesenchymal cells. Flow cytometry was employed to determine the percentages of EpCAM and Vimentin positive cells in the NEPT. The use of geneticin was helpful in preserving epithelial cells (~70% EpCAM positive cells) compared to the control (56% EpCAM positive cells) after a week in culture. Moreover we observed that a pancreatic media yielded the highest percentage of EpCAM positive cells (27%) and the lowest percentage of Vimentin positive cells (55%) compared to conventional media after 20 days in vitro.

  • Subjects / Keywords
  • Graduation date
    Fall 2011
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
  • Supervisor / co-supervisor and their department(s)
  • Examining committee members and their departments
    • MacDonald Patrick (Pharmacology)
    • Korbutt Gregory ( Surgery)
    • Churchil Thomas ( Surgery)
    • Adetola Adesida ( Surgery)