MOAP-1: A Candidate Tumor Suppressor Protein

• Author / Creator

  Law, Jennifer

• Modulator of apoptosis 1 (MOAP-1) is a BH3-like protein that plays a key role in death receptor-dependent apoptosis and cooperates with the tumor suppressor protein Ras association domain family 1A (RASSF1A) to promote Bax activation during cell death. Although loss of RASSF1A expression is frequently observed in human cancers, it is currently unknown if MOAP-1 expression may also be affected during carcinogenesis to result in uncontrolled malignant growth. Therefore, we sought to investigate the role of MOAP-1 in cancer development. Here, we demonstrate that MOAP-1 can effectively inhibit cell proliferation both in vitro and in vivo and undergoes frequent loss of expression during carcinogenesis. The ability of MOAP-1 to suppress tumor formation requires protein kinase C (PKC)-dependent non-degradative ubiquitination at lysine residue K278 and may involve the novel MOAP-1-interacting protein receptor for activated C-kinase 1 (RACK1). Our data indicate that MOAP-1 is a candidate tumor suppressor protein regulated by PKC-dependent ubiquitination.

• Subjects / Keywords

  • MOAP-1
  • RACK1
  • Apoptosis
  • Tumor Suppressor

• Graduation date

  Spring 2012

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R3V60P

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.