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The role of the Prader-Willi syndrome obesity protein, MAGEL2 in the proper functioning of circadian rhythm

  • Author / Creator
    Weselake, Sara Victoria
  • MAGEL2 is one of the five genes inactivated in Prader-Willi Syndrome (PWS), a genetic disorder, manifesting with symptoms of developmental delay and morbid obesity. Magel2 is highly expressed in the suprachiasmatic nucleus, which is the location of the central clock or circadian pacemaker. Magel2 knockout mice exhibit defects in circadian rhythm. I hypothesized that Magel2 plays a role in one of the inter-connecting feedback loops that control circadian rhythm in suprachiasmatic neurons. I determined that Magel2 acts as a repressive protein in the cycle’s feedback loop using a luciferase assay. Magel2 exerts this effect by restricting the movement of Bmal1 into the nucleus. Magel2 levels are then reduced by increasing Per2, associated with increase movement into the nucleus, as determined by experiments examining subcellular localization and effects on protein levels. Loss of Magel2 in PWS may contribute to sleep abnormalities in this disorder, specifically the cycling between different sleep stages.

  • Subjects / Keywords
  • Graduation date
    2010-11
  • Type of Item
    Thesis
  • Degree
    Sara Victoria Weselake
  • DOI
    https://doi.org/10.7939/R36W93
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Medical Sciences - Medical Genetics
  • Supervisor / co-supervisor and their department(s)
    • Wevrick, Rachel (Medical Genetics)
  • Examining committee members and their departments
    • Berry, Fred (Medical Genetics)
    • Godbout, Rosaline (Oncology)
    • McDermid, Heather (Medical Genetics, Biological Sciences)