Usage
  • 25 views
  • 133 downloads

Influences of grain boundaries and surface nanocrystallization of stainless Steel on Pseudomonas Aeruginosa Biofilm’s Adherence

  • Author / Creator
    Yu, Bin
  • A common complication associated with medical implants is the infectious bio-film, which can cause chronic infection that is difficult to control. Grain boundaries (GBs) in materials of medical implants are often preferential locations for bacteria to congregate, which could be attributed to higher affinity of grain boundaries for bacterial bio-films. In this study, the molecular interaction of the Pseudomonas aeruginosa receptor binding domain, a self-folding domain of 17 amino acid residues derived for the PilA structural protein, which can represents properties of Pseudomonas aerginosa biofilm, with microcrystalline stainless steel surfaces was examined with atomic force microscopy (AFM) both at grain boundaries and within grains. Adherence of Pseudomonas aeruginosa biofilm to nanocrystallized stainless steel surface was also determined using AFM. Results indicate that adherence of biofilm’ adherence at grain boundaries of microcrystalline surface is 2-fold higher than that of inside grains. Nanocrytalline surface is more resistant to biofilm than the microcrystalline one due to the formation of a strong oxidation film after annealing and thermal oxidation process. Surface nanocrystallization for enhanced corrosion resistance of Ag-incorporated 304 stainless steel surface was also studied. It is demontstrated that nanocrystallization of the antibacterial agent-incorporated stainless steel surface also provides an effective approach to control the corrosion problem resulting from the typical galvanic effect of multiphase alloys.

  • Subjects / Keywords
  • Graduation date
    2011-06
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3MP70
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Biomedical Engineering
  • Supervisor / co-supervisor and their department(s)
    • Randall T. Irvin, Department of Microbiology and Immunology
    • Dongyang Li, Department of Chemical and Materials Engineering, Department of Biomedical Engineering
  • Examining committee members and their departments
    • Dongyang Li, Department of Chemical and Materials Engineering
    • Larry Unsworth, Deapartment of Chemical and Materials Engineering, Biomedical Engineering
    • Randall T. Irvin, Department of Microbiology and Immunology