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Comparative proteomic and metabolomic analysis of a Staphylococcus variant (SG1) cultured in the presence and absence of butanol

  • Author / Creator
    Fu, Feifei
  • My research focuses on proteomic and metabolomic analysis of a solvent tolerant strain of Staphylococcus warneri called SG1 cultured in the presence and absence of 1-Butanol. On the proteomic analysis, the tryptic digests of SG1 were either directly analyzed by two-dimensional liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (2D-LC-ESI-QTOF MS) for protein identification or isotope labeled using dimethylation after guanidination (2-MEGA) followed by 2D-LC-ESI-QTOF MS for relative protein quantification. On the metabolomic analysis, the extracted metabolites were either labeled with 12C/13C dansyl chloride for relative quantitation of amine- and phenol-containing metabolites or labeled with 12C/13C p-dimethylaminophenacyl (DmPA) bromide for relative quantitation of carboxylic acid-containing metabolites. Finally, proteomic data and metabolomic data were combined and compared to help elucidate the solvent tolerant mechanism of SG1. The thesis work highlights the potential and significance of combining proteomic and metabolomic analyses for studying biological systems.

  • Subjects / Keywords
  • Graduation date
    2013-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R33X1G
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Chemistry
  • Supervisor / co-supervisor and their department(s)
    • Weiner, Joel (Department of Biochemistry)
    • Li, Liang (Department of Chemistry)
  • Examining committee members and their departments
    • Weiner, Joel (Department of Biochemistry)
    • Li, Liang (Department of Chemistry)
    • Le, Chris (Department of Laboratory Medicine and Pathology)