Dengue NS1 Detection using Chemically Modified Silicon Micropillars

• Author / Creator

  Singh,Minashree

• Dengue fever is a mosquito born viral disease that is reaching epidemic proportions. Despite of various control means, it is still flourishing and is becoming a major health concern around the world. In this thesis, a novel diagnosis method for the detection of Dengue virus has been developed. Micro-spot-integrated pillars chip (MSIP) has been used to increase the surface area for the immunoreaction and hence the probability of the Dengue virus detection. The MSIP chip used for the diagnosis of Dengue NS1 was chemically modified to immobilize the capture antibodies. The surface modification and the biofunctionalization of the capture antibody were done to increase the robustness and surface availability of the capture antibodies for the antibody-antigen reaction. Each step of the surface modification and biofunctionalization process was characterized by using different tools. The result showed that the method developed was five fold more sensitive than the conventional techniques.

• Subjects / Keywords

  • Dengue
  • Covalent immobilization
  • Micropillars chip based immunoassay
  • Silicon micropillars

• Graduation date

  Fall 2012

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R3GT6R

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.